应用拮抗和ISSR方法综合鉴定灰树花菌株

Identification of Grifola frondosa by Antagonistic Test and ISSR

【目的】准确、科学地鉴定灰树花菌株,明确其亲缘关系。【方法】采用拮抗和ISSR分子标记对供试的11个灰树花菌株进行鉴定和遗传多样性分析。【结果】拮抗反应表明,供试菌株之间拮抗作用有一定差异,根据拮抗反应的有无,供试菌株分为4组。ISSR分子标记分析表明,当相似性系数达到0.87水平时,供试菌株聚成5组,第1组包括菌株1 (石家庄灰树花)、2 (台灰)、10 (XG-W)、5 (Gr0001+2)、6 (CN)、7 (雪圆);第2组为菌株11 (CN-9);第3组为菌株4 (Gr0003);第4组包括菌株8 (H)、9 (Hokto);第5组为菌株3(Gr20)。【结论】拮抗反应与ISSR两种方法结论较一致,推测石家庄灰树花与台灰,Gr0001+2、CN与雪圆,H与Hokto这3组菌株分别来源于同一个菌株,属于同种异名现象Gr0003、H和Hokto亲缘关系较远;Gr20与其他菌株亲缘关系最远。

【Objective】To reliably identify strains of Grifola frondosa and precisely determine the genetic relationship among them.【Method】The identification and genetic diversity analysis on 11 strains of G. frondosa were carried out by the combined use of the antagonistic challenging test and ISSR.【Result】The antagonistic test on the strains demonstrated apparent differences on their responses. Presence or absence of antagonism allowed the classification of the strains into 4 distinct groups. Meanwhile,from the ISSR analysis,when the similarity coefficient reached 0.87,the strains were clustered into 5 groups. They were Group 1 that included Strain #1 (Shijiazhuang ash tree flower),Strain #2 (stage ash),Strain #10 (XG-W),Strain #5 (Gr0001+2),Strain #6 (CN),and Strain #7 (snow circle);Group 2,Strain #11 (CN-9);Group 3,Strain #4 (Gr0003);Group 4,Strain #8 (H) and Strain #9 (Hokto);and,Group 5,Strain #3 (Gr20).【Conclusion】It appeared that Strain #1 and Strain #2 belonged to a same species;so were Strains #5,Strain #6 and Strain #7,and Strains #8 and Strain #9. At the similarity coefficient of 0.8,Strain #4 could be clustered with Strain #8 and Strain #9 with slight differences indicating a distance on genetic relationship among them. Strain #3 had a lowest coefficient of 0.34,therefore,it was most remotely related to all.