PTEN与TGF-β1在上皮性卵巢癌中的表达及临床意义

Expression of PTEN and TGF-β1 in Epithelial Ovarian Cancer and Their Clinical Significance

目的探讨第10号染色体缺失的磷酸酶和张力蛋白同源物基因(phosphatase and tension homolog deleted on chromosome ten,PTEN)、转化生长因子β1(transforming growth factor β1,TGF-β1)在上皮性卵巢癌患者中的表达及临床意义。方法选取我院2015年1月—2017年12月收治的上皮性卵巢癌100例作为上皮性卵巢癌组,另选取同期收治的卵巢良性肿瘤40例作为卵巢良性肿瘤组。采用免疫组织化学染色法分析PTEN与TGF-β1在上皮性卵巢癌和卵巢良性肿瘤组织中的表达情况,并探讨PTEN、TGF-β1与上皮性卵巢癌病情程度的关系以及二者阳性表达的相关性。结果PTEN在上皮性卵巢癌组织中的阳性表达率为38.00%低于卵巢良性肿瘤组织中的80.00%(P<0.01);TGF-β1在上皮性卵巢癌组织中的阳性表达率为76.00%明显高于卵巢良性肿瘤组织中的40.00%(P<0.01)。TGF-β1在Ⅲ~Ⅳ期上皮性卵巢癌组织中的阳性表达率为88.24%高于Ⅰ~Ⅱ期上皮性卵巢癌组织中的50.00%(P<0.01);PTEN在Ⅰ~Ⅱ期上皮性卵巢癌组织中的阳性表达率为65.62%明显高于Ⅲ~Ⅳ期上皮性卵巢癌组织中的25.00%,在有淋巴结转移的上皮性卵巢癌组织中的阳性表达率为28.12%低于无淋巴结转移的上皮性卵巢癌组织中的55.56%,差异均有统计学意义(P<0.01)。PTEN与TGF-β1在上皮性卵巢癌组织中的表达呈负相关(r=-0.235,P=0.018)。结论TGF-β1参与上皮性卵巢癌的发生,并与上皮性卵巢癌病情进展密切相关。TGF-β1过度表达伴随PTEN表达下降或部分缺失,与上皮性卵巢癌的发生发展、细胞耐药密切相关。

Objective To investigate the expressions of phosphatase and tensin homolog deleted on chromosome 10 (PTEN), and transforming growth factor β1(TGF-β1) in patients with epithelial ovarian cancer (OvCa) and their clinical significance. Methods A total of 100 cases with epithelial OvCa admitted to our hospital from January 2015 to December 2017 were selected as the epithelial OvCa group, and 40 cases with benign ovarian tumors admitted during the same period were selected as the benign ovarian tumor group. Immunohistochemical staining (immunohistochemistry) was used to analyze the expressions of PTEN and TGF- 1 in epithelial OvCa and benign ovarian tumor tissues, and to explore the relationship between PTEN and TGF- 1 and the severity of epithelial OvCa and the correlation between their positive expressions. Results The positive expression rate of PTEN in epithelial OvCa tissue was 38.00%, lower than that in benign ovarian tumor tissue (80.0%, P <0.01). The positive expression rate of TGF-β1 in epithelial OvCa tissue was 76.00%, significantly higher than that of 40.00% in the benign ovarian tumor tissue ( P <0.01). In stage Ⅲ-Ⅳ epithelial OvCa tissue, the positive expression rate was 88.24%, which was higher than that (50.00%) of stageⅠ-Ⅱ( P < 0.01). Positive expression rate of PTEN in stageⅠ-Ⅱ epithelial OvCa was 65.62%, which was significantly higher than that (25.00%) in stage Ⅲ-Ⅳ epithelial OvCa tissue. In epithelial OvCa tissues with lymph node metastasis, the positive expression rate was 28.12%, which was lower than 55.56% without lymph node metastasis ( P <0.01). PTEN was negatively correlated with the expression of TGF-β1 in epithelial OvCa ( r =-0.235, P =0.018). Conclusion TGF-β1 is involved in the development of epithelial OvCa and is closely related to the progression of epithelial OvCa. Overexpression of TGF-β1 is associated with decreased expression or partial deletion of PTEN, which is closely related to the occurrence and development of epithelial OvCa as well as drug resistance.