miR-424-5p过表达对乳腺癌细胞增殖和迁移能力的影响

Effects of miR-424-5p expression on proliferation and migration of breast cancer cells

目的探讨miR-424-5p过表达对乳腺癌细胞增殖和迁移能力的影响。方法体外传代培养人乳腺癌细胞MCF-7(以下称MCF-7细胞)。取传3代、对数生长期、生长状态良好的MCF-7细胞,随机分为miR-424-5p组、阴性对照组、空白对照组,miR-424-5p组、阴性对照组分别转染miR-424-5p mimics、miRNA negative control,空白对照组不予转染。收集各组转染48h细胞,采用real-time PCR法检测miR-424-5p表达;采用MTT法检测转染48h再培养0、24、48、72h细胞增殖能力,采用细胞划痕实验检测转染48h再培养24h细胞迁移能力。结果miR-424-5p组miR-424-5p相对表达量明显高于阴性对照组和空白对照组(P均<0.05),而阴性对照组与空白对照组比较P>0.05。miR-424-5p组转染48h再培养72h细胞增殖能力明显低于阴性对照和空白对照组(P均<0.01),而三组转染48h再培养0、24、48h组间两两比较P均>0.05。miR-424-5p组细胞迁移能力明显低于阴性对照组和空白对照组(P均<0.01),而阴性对照组与空白对照组比较P>0.05。结论miR-424-5p过表达能抑制乳腺癌细胞增殖和迁移能力。miR-424-5p有可能成为治疗乳腺癌的一个潜在靶点。

ObjectiveTo investigate the effects of miR-424-5p on the proliferation and migration of MCF-7 breast cancer cells. Methods The human breast cancer cell line MCF-7 (hereinafter referred to as MCF-7 cells) was cultured in vitro. MCF-7 cells with good growth were randomly divided into the miR-424-5p group, negative control group, and blank control group.The cells in the miR-424-5p group and negative control group were transfected with miR-424 mimics and miRNA negative control, respectively, and the blank control group was not transfected. At 48 h after transfection, the cells of each group were collected, and the expression of miR-424-5p was detected by real-time PCR. After 48-hour transfection, the cells were then cultured for 0, 24, 48, and 72 h, respectively, and the proliferation of each group was detected by MTT. MCF-7 cells were transfected for 48 h and cultured for 24 h, and cell scratching assay was used to detect the migration ability of each group. Results The relative expression of miR-424-5p in the miR-424-5p group was significantly higher than that in the negative control group and the blank control group (P<0.05), while no significant difference was found between the negative control group and the blank control group (P>0.05). The proliferation of MCF-7 cells transfected with miR-424-5p mimics for 72 h in the decreased significantly as compared with those of the negative control group and the blank control group;when the cells were cultured for 0, 24, 48, and 72 h after 48-hour transfection in the three groups, the difference was not statistically significant (P>0.05). The migration ability of the miR-424-5p group was significantly lower than that of the negative control group and the blank control group (P<0.01), while no significant difference was found between the negative control group and the blank control group (P>0.05). Conclusion Overexpression of miR-424-5p can inhibit the proliferation and migration of breast cancer cells, and miR-424-5p may become a potential target for treatment of breast cancer.