大鼠早期急性胰腺炎外周血及胰腺组织中PTEN表达水平及其意义的研究

Expression of PTEN in Peripheral Blood and Pancreatic Tissues in Rats with Acute Pancreatitis at Early Stage and the Significance

目的探索PTEN在大鼠急性胰腺炎早期外周血及胰腺组织的变化及其意义。方法将36只大鼠随机分至A、B、C三组,每组十二只。A组采用经典4%的牛黄胆酸钠逆行胰胆管注射制造大鼠急性胰腺炎模型,B组为假手术组,C组在建立急性胰腺炎基础上采用腹腔置管引流干预。检测记录三组血清中淀粉酶、脂肪酶、PTEN的变化,并做胰腺病理评分。用免疫组织化学法检测胰腺组织中的PTEN的表达。结果在A组和C组,血清淀粉酶、脂肪酶于B组(P<0. 05),胰腺病理评分A组和C组高于B组(P<0. 05),胰腺病理评分A组高于C组(P<0. 05);A组血清及胰腺组织中的PTEN高于B组和C组,C组表达水平高于B组(P<0. 05)。结论PTEN可能作为大鼠急性胰腺炎病情严重程度早期预测的指标。

Objective To investigate the variations of phosphatase and tensin( PTEN) expression in the peripheral blood and pancreatic tissues in rats with acute pancreatitis at early stage. Methods 36 rats were divided into 3 groups:group A( blank control group),group B( sham operation group) and group C( observation group),12 in each;acute pancreatitis was induced in rats in group A and group C by retrograde pancreatic duct injection of 4. 0% sodium taurocholate while intervention with abdominal drainage was added to rats in group C;the levels of serum amylase,lipase and PTEN in all the rats were detected and the variations were recorded,and pathological evaluation was made;immunohistochemistry was applied in detecting the expression of PTEN in pancreatic tissues. Results The levels of amylase and lipase in group A and group C were higher than those in group B( P<0. 05);the pancreatic pathological scores in group A was higher than those in group C( P < 0. 05);the expression of PTEN in serum and pancreatic tissues was higher in group A than that in group B and in group C while it was higher in group C than that in group B( P<0. 05).Conclusions PTEN may be taken as an early predicative index for the severity of acute pancreatitis in rats.