LncRNA PVT1在结直肠癌细胞增殖、侵袭和转移中的作用

Role of LncRNA PVT1 in proliferation,invasion and metastasis of colorectal cancer cells

目的探讨长链非编码RNAs(lncRNAs)浆细胞瘤多样异位基因1(PVT1)在结直肠癌细胞增殖、侵袭和转移中的作用。方法结直肠癌细胞株HT-29分为三组,干扰PVT1(si-PVT1)组转染si-PVT1,干扰对照(si-NC)组转染si-NC,空白组不进行转染。采用CCK-8法检测24h、48h细胞增殖,流式细胞术检测细胞凋亡,划痕实验检测细胞迁移,Transwell小室检测细胞侵袭。结果转染后24h、48h,与空白组和si-NC组相比,si-PVT1组的LncRNAPVT1表达量显著减少(P<0.05),细胞增殖抑制率显著降低(P<0.05),细胞凋亡率显著降低(P<0.05),细胞划痕愈合率、细胞侵袭率都显著增加(P<0.05)。结论LncRNAPVT1在结直肠癌中可发挥抑癌基因的作用,抑制LncRNAPVT1的表达从而促进结直肠癌细胞增殖、侵袭和转移,抑制细胞凋亡。

Objective To investigate the role of long-chain non-coding RNAs (LncRNA plasmacytoma variant translocation (PVT1) in the proliferation,invasion and metastasis of colorectal cancer cells. Methods Colorectal cancer cell line HT-29 was divided into three groups. Si-PVT1 group was transfected with si-PVT1,si-NC group was transfected with si-NC,and blank group was not transfected. Cell proliferation was detected by CCK-8 method,apoptosis were detected by flow cytometry,cell migration were detected by scratch test,and cell invasion were detected by Transwell chamber. Results At 24 h and 48 h after transfection,the expression of LncRNA PVT1 were significantly decreased in the si-PVT1 group compared with the si-NC group ( P <0.05),and the cell proliferation inhibition rates were significantly decreased ( P <0.05). The apoptotic rates were significantly decreased ( P <0.05),and the cell scratch healing rate and cell invasion rate were significantly increased ( P <0.05). Conclusion LncRNA PVT1 can play a role as a tumor suppressor gene in colorectal cancer. Inhibition of LncRNA PVT1 expression can promote the proliferation,invasion and metastasis of colorectal cancer cells and inhibit cell apoptosis.