房山区4种动物源性成分PCR检测试验

Research on the PCR Test of Four Species of Animal Derived Materials in Fangshan District, Beijing

本研究利用牛、羊、猪、鸡线粒体mtDNA中保守序列16srRNA,设计一对动物的通用引物,检测了含有动物源性成分的样品,同时设计了针对牛、羊、猪、鸡mtDNA中的保守序列,选择4对特异性引物,扩增得到目的片段大小分别为271bp、274bp、212bp和266bp。利用通用引物,优化了PCR反应体系及其反应条件,建立了从未知样品中快速获取动物源性成分检测的PCR方法;利用牛、羊、猪、鸡特异性引物,建立了PCR方法快速鉴定牛、羊、猪、鸡这4种动物源性成分的方法。

A general primer was designed according to 16S rRNA gene sequence of cattle, sheep, pigs and chicken mtDNA in order to detect animal derived materials in foodstuff. Three species-specific primers were designed according to conserved sequence of mtDNA of cattle, sheep, pigs, and chicken were used for amplification of 271, 274 , 212 and 266 bp fragments respectively. PCR detection of animal derived materials in unknown foodstuff was developed by using general primer and optimizing relevant system and PCR condition. Also PCR detection method of cattle, sheep, pigs and chicken was designed by applying species-specific primers.