江西省赣州市蜱携带立克次体和埃立克体的调研

Rickettsia and Ehrichia from Ticks: field and laboratory investigation in Ganzhou City, Jiangxi Provence, China

目的 了解江西省赣州市蜱的种类及其携带的病原体,为蜱传疾病的防控提供科学依据。方法 在野外用布旗法和在动物体表采集蜱,进行种类鉴定。用PCR法扩增立克次体(Rickettsia sp.)gltA基因和埃立克体(Ehrlichia sp.)16S rRNA基因片段并测序,进行系统进化分析。结果 江西省赣州市4县采集的395只蜱的种类包括2属4种,分别为血蜱属的长角血蜱(Haemaphysalislongicornis)、嗜群血蜱(H.concinna)、具角血蜱(H.cornigera)和扇头蜱属的微小扇头蜱(Rhipicephalus microplus)。395只蜱分为43批检测立克次体和埃立克体,共有18批阳性。系统进化分析显示,龙南县22批蜱中检测到16批阳性,来源于具角血蜱的LN1615株与扇头蜱立克次体(R.rhipicephali)处于同一分支,来源于微小扇头蜱的LN1620株与马赛立克次体(R.massiliae)处于同一分支,其余的12株来源于具角血蜱和2株来源于微小扇头蜱,与日本立克次体(R.japonica)处于同一分支。崇义县CY1602株来源于嗜群血蜱与R.raoultii处于同一分支。于都县YD1606株为来源于长角血蜱的埃立克体,与2010年Yonaguni206(HQ697589)、Yonaguni138(HQ697588)和2009年HLAE178(GU075695)处于同一分支。安远县微小扇头蜱检测均为阴性。结论 本文对江西省赣州市采集的蜱进行了鉴定,首次在江西省赣州市的蜱中检测到日本立克次体、扇头蜱立克次体、马赛立克次体、R.raoultii和埃立克体,为江西省蜱种类的调查及其传播疾病的预防控制提供根据。

To understand the species of ticks and the pathogens they carry in Ganzhou City,Jiangxi Province,and to provide scientific basis for the prevention and control of tick-borne diseases.Ticks were collected in the field by flag cloth method and on the surface of animals,and the species were identified.The gltA gene and the 16s rRNA gene fragment of Rickettsia sp.and Ehrlichia sp.were amplified and sequenced by PCR,and the phylogenetic analysis was carried out.The species of 395 ticks collected from 4 counties of Ganzhou City,Jiangxi Province included 2 genus and 4 species,which were Haemaphysalislongicornis,H.concinna,H.cornigera,Rhipicephalusmicroplus respectively.Three hundred and ninety-five ticks were divided into 43 batches to detect Rickettsia sp.and Ehrlichia sp.A total of 18 ticks were positive.Phylogenetic analysis showed that 16 batches of ticks were positive in 22 batches of ticks in Longnan County,and LN1615 strains from H.cornigera were in the same branch as R.rhipicephali.The LN1620 strain from Rhipicephalusmicroplus was in the same branch as R.massiliae,while the other 12 strains came from H.cornigeras and 2 strains from Rhipicephalusmicroplus,which were in the same branch as R.japonica.The CY1602 strain in Chongyi County originated from H.concinna and R.raoultii was in the same branch.The YD1606 strain of Yudu County is Ehrlichia sp.from Haemaphysalislongicornis,which is in the same branch as Yonaguni206(HQ697589),Yonaguni138(HQ697588)and HLAE178(GU075695)in 2009.The detection of Rhipicephalusmicroplus ticks in Anyuan County was negative.The ticks collected were identified in this paper.R.japonica,R.rhipicephali,R.massiliae,R.raoultii and Ehrlichia sp.were detected for the first time in Ganzhou City,Jiangxi Province.It provides the basis for the investigation of ticks in Jiangxi Province and the prevention and control of transmitted diseases.