含模板结构分子印迹材料制备及识别性能研究

Preparation of Molecularly Imprinted Materials Containing Template Structure and Its Recognition Property

以双酚A(BPA)为模板分子,α-甲基丙烯酸(MAA)为功能单体,以含模板分子结构的双酚A二甲基丙烯酸酯(BPDMA)为交联剂,制备了含模板结构的分子印迹聚合物材料,并与以二甲基丙烯酸乙二醇酯(EGDMA)和N,N-亚甲基双丙烯酰胺(BIS)为交联剂制备的BPA印迹材料进行对比。结果表明:以BPDMA为交联剂制备的材料印迹性能最佳,其印迹因子为5.32,吸附量为5.05 mg/g;以EGDMA和BIS为交联剂制备的材料印迹因子分别为1.41和1.45,吸附容量分别为0.96 mg/g和2.79 mg/g。将所制备材料应用于牛奶中BPA的富集检测,得到的加标回收率为95.0%~98.9%,相对标准偏差为3.5%~4.2%,表明得到的印迹材料可用于复杂体系BPA的选择性富集。

Crosslinker is one of the most important factors that dominate the recognition performance of molecularly imprinted polymer(MIP).In this research,MIP was prepared by using bisphenol A(BPA)as the template molecule,α-methacrylate(MAA)as the functional monomer and bisphenol A dimethylacrylate(BPDMA)as the crosslinker,respectively.The resulted MIP was also compared with those obtained by using dimethyl glycol acrylate(EGDMA)and N,Nmethylene diacrylamide(BIS)as the crosslinking agents,respectively.The results indicated that the MIP material prepared using BPDMA as the crosslinker gave rise to the best imprinting factor(5.32)and adsorption capacity(5.05 mg/g),which were far superior to those already reported BPA imprinted materials.The imprinting factors of the materials prepared using EGDMA and BIS as the crosslinking agents were 1.41 and 1.45,respectively,and the adsorption capacities were 0.96 mg/g and 2.79 mg/g,respectively.Thus,cross-linker plays an important role in the recognition property of MIP.The resulted MIP was further applied to enrich BPA from milk.The recovery rate was 95.0%-98.9%and the relative standard deviation was 3.5%-4.2%.These results indicated that the prepared imprinted material could be used for the selective enrichment of BPA from complex systems.