丹参叶片离体培养体系的建立与优化

Establishment and optimization of in vitro culture system of Salvia miltiorrhiza leaves

以丹参叶片为外植体,利用正交试验等方法筛选优化丹参叶片不定芽诱导、不定芽丛增殖及壮苗生根、不定根毛状根诱导的适宜方案。结果表明:适宜叶片不定芽诱导的配方是MS+0.5mg/L6-BA+30g/L蔗糖+琼脂6.5g/L;不定芽丛增殖的最适配方是2/3MS+0.5mg/L6-BA+0.2mg/LGA3+30g/L蔗糖+6.5g/L琼脂,平均芽增殖系数达12.00;适宜幼苗产生根的配方是1/2MS+0.05mg/L2,4-D+0.1mg/LIBA+20g/L蔗糖+7g/L琼脂,生根率达100%;适宜叶片诱导不定根毛状根的配方是MS+0.5mg/L2,4-D+30g/L蔗糖+6.5g/L琼脂。