超声波协同复合酶法提取栀子中黄酮的研究

Study on extraction of flavonoids from gardenia by ultrasonic-assisted complex enzymatic method

采用超声波协同复合酶法提取栀子中黄酮类物质,采用正交试验对提取条件进行优化。结果表明:最佳提取工艺为酶质量浓度0.8%、酶解温度45℃、酶解时间90min、提取液pH4.8,在此条件下,提取率高达3.6%。采用DPPH法判定黄酮的活性,采用紫外分光光度法测定提取的黄酮类物质的纯度,纯度为97.6%。

Flavonoids from gardenia was extracted by ultrasonic-assisted complex enzymatic method.The extraction conditions were optimized by orthogonal test.The results showed that the optimum extraction process were as follows:enzyme dosage 0.8 %(mass concentration),extraction temperature 45℃,time 90 min,pH 4.8,under these conditions,the extraction rate was up to 3.6 %.The DPPH method was used to determine the activity of flavonoids.The purity of the flavonoids was 97.6% deterined by UV spectrophotometry.