摘要
目的观察切除大鼠不同卵巢体积对其动情周期及体质量的影响。方法 67只10周龄SD大鼠适应1周后,按照切除不同的卵巢体积共分为6组:A组,假手术组(n=12);B组,切除单侧1/2(n=12);C组,切除单侧全部(n=12);D组,切除单侧全部+对侧1/2(n=12);E组,切除单侧全部+对侧3/4(n=12);F组,切除双侧全部(n=7)。卵巢部分切除术后4、8、12周连续9 d监测大鼠阴道细胞涂片,并于手术当天、术后4、6、8、10、12周监测大鼠体质量。分析切除大鼠不同卵巢体积对其动情周期及体质量的影响。结果 E组以及F组大鼠的动情周期与其余4组间差异有统计学意义(P<0.05),动情周期天数高于其余4组。F组大鼠体质量明显高于其余5组(P<0.05)。结论大鼠卵巢切除超过单侧全部+对侧3/4组时对动情周期有影响,双侧全切对体质量有影响。
Objective To investigate the effect of different ovarian tissue volume on estrous cycle and body weight in rats.Methods Totally 67 10-week-old Sprague-Dawley rats were divided into 6 groups according to removed different ovarian tissue volume after one week’s acclimatization.Group A,sham operation group(n=12),Group B,the half of left ovary removed(n=12),Group C,the left whole ovary(n=12),Group D,the left whole ovary and the half of right ovary removed(n=12),Group E,the left whole ovary and the three quarters of right ovary removed(n=12),Group F,both left and right ovaries removed(n=7).The vaginal smear of the rats was monitored for 9 consecutive days at 4 weeks,8 weeks,and 12 weeks after partial ovarian resection,and the body weight of the rats was monitored on the day of surgery,4 weeks,6 weeks,8 weeks,10 weeks,and 12 weeks after surgery.The effects of the ovarian volumes on the estrous cycle and body weight of the rats were analyzed.Results There was a statistically significant difference in estrous cycle between the E group and the group and the other 4 groups(P<0.05),and the estrous cycle days of Group E,Group F were higher than that of the other 4 groups.Rats of weight in Group F is higher than that in the other 5 groups(P<0.05).Conclusion Removed one and three quarters of ovary imposed an effect on the rat’s estrous cycle,and bilateral oophorectomy imposed an effect on rat’s body weight.
作者
程姣姣
阮祥燕
周琦
李扬璐
杜娟
金凤羽
谷牧青
Alfred O.Mueck
Cheng Jiaojiao;Ruan Xiangyan;Zhou Qi;Li Yanglu;Du Juan;Jin Fengyu;Gu Muqing;Alfred O.Mueck(Department of Gynecological Endocrinology,Beijing Obstetrics and Gynecology Hospital,Capital Medical University,Beijing 100026,China;Department for Women's Health,University Women's Hospital and Research Center for Women's Health,University of Tuebingen,Tuebingen D-72076,Germany;Department of Gynecology,Beijing Obstetrics and Gynecology Hospital,Capital Medical University,Beijing 100026,China;Department of Reproductive Medicine,Beijing Obstetrics and Gynecology Hospital,Capital Medical University,Beijing 100026,China)
出处
《首都医科大学学报》
CAS
北大核心
2019年第4期554-559,共6页
Journal of Capital Medical University
基金
首都临床特色应用研究与成果推广项目(Z161100000516143)
北京市医院管理局“登峰”计划专项经费资助(DFL20181401)
北京市医院管理局临床技术创新项目(XMLX201710)
首都卫生发展科研专项(2016-2-2113)
国家外专局引智重点项目(20181100005)
医疗健康技术创新与人才培养专项基金(2017041900004,2018042000001)
北京市卫生系统高层次卫生技术人才项目(2014-2-016)~~
关键词
卵巢体积
动情周期
卵巢组织冻存
早发性卵巢功能不全
ovarian volume
estrous cycle
ovarian tissue cryopreservation
premature ovarian insufficiency