LncRNA SChLAP1调节前列腺癌的凋亡作用及其机制研究

Study on the role of LncRNA SChLAPl in regulating apoptosis of prostate cancer and its mechanism

目的研究LncRNASChLAP1调节前列腺癌细胞凋亡的可能分子机制。方法采用慢病毒转染前列腺癌PC-3细胞,并用qPCR检测转染率;利用凋亡检测试剂盒检测转染PC-3细胞的凋亡情况,通过生物信息学确定其下游的可能靶点。结果在不同靶点病毒作用下PC-3细胞可以下调SChLAP1的表达,其中3#位点的下调效率最大达40%。PC-3细胞下调后,与阴性对照组相比细胞凋亡比例明显上升,差异有统计学意义。结论SChLAP1可以调节前列腺癌细胞的凋亡,而miR-101-5p是其下游的靶点之一。

Objective To investigate the possible molecular mechanism of LncRNA SChLAP1 in the regulation of apoptosis in prostate cancer cells. Methods Prostate cancer PC-3 cells were transfected with lentivirus, and the transfection rate was detected by qPCR. The apoptosis of PC-3 cells was detected by apoptosis detection kit, and the possible downstream targets were determined by bioinformatics. Results PC-3 cells could down-regulate the expression of SChLAP1 under the action of different target viruses, and the down-regulation efficiency of 3# locus was up to 40%. After PC-3 cells were down-regulated, the proportion of apoptosis was significantly higher than that of the negative control group, and there was a statistical difference. Conclusion SChLAP1 can regulate the apoptosis of prostate cancer cells, and miR-101-5p is one of its downstream targets.