金属硫蛋白2A、E钙黏蛋白及细胞周期蛋白E与前列腺癌生化复发的相关性

Correlation of metallothionein-2A, E-cadherin and cyclin E with biochemical recurrence in prostate cancer

目的探讨金属硫蛋白2A(MT-2A)、E钙黏蛋白(E-cadherin)、白细胞介素6(IL-6)、细胞周期蛋白E(cyclin E)、增殖细胞核抗原(PCNA)和抗凋亡蛋白bcl-2在前列腺癌组织中的表达及其与前列腺癌生化复发之间的相关性。方法收集2012年10月至2017年10月于山西大医院接受前列腺癌根治性手术的128例患者的前列腺癌组织标本,记录患者的临床数据。制作组织芯片,采用免疫组织化学生物素-亲和素复合物(ABC)染色法检测芯片组织MT-2A、E-cadherin、IL-6、cyclin E、PCNA和bcl-2的表达水平,并分析不同分子标志物与前列腺癌生化复发之间的相关性。结果 128例前列腺癌患者生化复发率为30.5%(39/128),低危、中危和高危前列腺癌患者生化复发率分别是14.8%(8/54)、38.7%(24/62)和58.3%(7/12)。前列腺癌患者危险分级和病理学T分期与MT-2A、cyclin E、IL-6、E-cadherin表达均有关(均P<0.05)。多因素Cox风险模型结果显示,高危险分级(HR=1.81,95% CI 1.56~2.19,P=0.042)、MT-2A阳性表达(HR=2.01,95% CI 1.08~3.15,P=0.005)、cyclin E阳性表达(HR=1.79,95% CI 1.08~2.21,P=0.042)和E-cadherin阴性表达(HR=1.92,95% CI 1.22~2.45,P=0.020)是前列腺癌生化复发的独立危险因素。结论前列腺癌组织中MT-2A、cyclin E和E-cadherin是预测前列腺癌生化复发的重要分子标志物。

Objective To investigate the expressions of metallothionein-2A (MT-2A), E-cadherin, interleukin-6 (IL-6), cyclin E, proliferating cell nuclear antigen (PCNA) and bcl-2 in prostate cancer tissues and their correlation with biochemical recurrence of prostate cancer. Methods Tissue specimens from 128 cases of prostate cancer who underwent radical prostatectomy in Shanxi Dayi Hospital from October 2012 to October 2017 were processed and transferred into tissue microarrays, the clinicopathological parameters of patients were also recorded. The expression levels of MT-2A, E-cadherin, IL-6, cyclin E, PCNA and bcl-2 were detected by immunohistochemical avidin-biotin complex (ABC) staining. The correlation between different molecular markers and biochemical recurrence of prostate cancer was analyzed. Results The biochemical recurrence rate of 128 patients with prostate cancer was 30.5%(39/128). The biochemical recurrence rates of low-risk, intermediate-risk and high-risk prostate cancer patients were 14.8%(8/54), 38.7%(24/62) and 58.3%(7/12), respectively. The risk classification and pathological T stage of patients with prostate cancer were associated with the expressions of MT-2A, cyclin E, IL-6 and E-cadherin (all P < 0.05). Multivariate Cox risk model showed that the high risk classification (HR=1.81, 95% CI 1.56-2.19, P=0.042), MT-2A positive expression (HR=2.01, 95% CI 1.08-3.15, P=0.005), cyclin E positive expression (HR=1.79, 95% CI 1.08-2.21, P=0.042) and E-cadherin negative expression (HR=1.92, 95% CI 1.22-2.45, P=0.020) were the independent risk factors for biochemical recurrence of prostate cancer. Conclusion The expression of MT-2A, cyclin E and E-cadherin may serve as independent predictors for biochemical recurrence of prostate cancer.