山茶亚属植物DNA傅立叶红外光谱分析

Fourier Infrared Spectroscopic Analysis of Plants DNA from Genus Camellia

以山茶亚属77种茶花物种为实验材料,采用改良CTAB法提取植物基因组DNA,使用傅立叶红外光谱数据系统聚类数学模型进行山茶亚属亲缘关系分析。选取2000~500cm^-1波段的红外光谱原始数据、标准化数据、一阶导数、二阶导数、1700~700cm^-1波段二阶导数、二阶导数主成分进行系统聚类分析。研究结果显示:(1)所提山茶亚属植物DNA分子在1760~1480cm^-1、1440~1330cm^-1、1250~1000cm^-1、1000~800cm^-1峰位均有典型的DNA傅立叶红外光谱特征峰。(2)一阶导数系统聚类总辅助率为42.9%,实验样品分为四个组,系统聚类效果相对较佳;原始数据系统聚类总辅助率为54.3%,实验样品被分为三个组;二阶导数主成分系统聚类总辅助率为22.1%,实验样品分为四个组,但有很多样品无法分组;二阶导数1700~700cm^-1波段系统聚类总辅助率为53.2%,实验样品仅能分为两个组;标准化数据与二阶导数系统聚类结果,实验样品分不开,只能作为一个组,效果不佳。结论,植物基因组DNA傅立叶红外光谱数据系统聚类法对山茶亚属亲缘关系分析有辅助作用。

The genetic relationship and species identification of 77 species from camellia subgenus were analyzed by using Fourier infrared spectroscopy of plant genome DNA extracted by modified CTAB method. The peaks between 1250 and 1000 cm^-1 are about the vibration information of the main chain phosphate – five carbon sugar skeletons of the nucleic acid. The peaks in range of 1000-800 cm^-1 reflect the five-carbon sugar information. The peaks between 1440 cm^-1 and 1330 cm^-1 are about glycosidic bond rotation. The peaks between 1760 cm^-1 and 1480 cm^-1 reflects the base pairs and base stacki of the nucleic acid. Hierarchical clustering analysis model were built by using original data, standardization of data, the first derivative, second derivative, the principal component of second derivative between 2000-500 cm^-1, second derivative between 1700 and 700 cm^-1. The accuracy of original data system clustering was 54.3%;the experiment samples were divided into three groups. The hierarchical cluster of standardized data can't be grouped correctly;the accuracy of first derivative system clustering was 42.9%;the experimental samples are divided into four groups;clustering accuracy of the second derivative system can't be grouped correctly, only as a group. The accuracy of principal component system clustering of second derivatively was 22.1%;the experimental samples are divided into four groups,but there are many samples that cannot be grouped or can only be grouped confusedly. The accuracy second derivative system clustering between 700-1700 cm^-1 was 53.2%;the experimental samples are divided into two groups. By comparison, the first derivative system clustering analysis is the best as the samples cluster better and the clustering accuracy is 42.9%. Our conclusion is that Fourier infrared spectrum analysis of plant genome DNA from camellia subgenus has a supplementary role on genetic relationship analysis.