miR-34a及靶蛋白Foxp1对寻常型银屑病的作用研究

Regulation Effect of MiR-34a and Its Target Foxp1 Protein in Psoriasis Vulgaris

目的探讨miR-34a其靶蛋白Foxp1调控在寻常型银屑病发生过程中其表达水平和作用意义.方法运用实时荧光定量PCR法检测miR-34a在12例寻常型银屑病患者皮损组织和12例正常健康皮肤组织中的表达水平.利用化学合成的miR-34a mimic、miR-34a inhibitor,转染HaCaT细胞,使其过表达或抑制表达miR-34a;采用免疫印迹技术检测转染细胞中Foxp1蛋白的表达.结果寻常型银屑病患者皮损组织miR-34a的表达水平显著高于正常健康皮肤组织,差异有统计学意义(t=3.047,P<0.05).免疫印迹检测转染后HaCaT细胞中Foxp1蛋白结果表明:过表达miR-34a时Foxp1蛋白表达量增加,miR-34a抑制表达时Foxp1蛋白表达降低,差异有统计学意义(F =286.722,P<0.01).结论 miR-34a在寻常型银屑病患者皮肤组织中呈高表达,Foxp1作为miR-34a的靶蛋白受其调控参与寻常型银屑病的发生、发展过程.

Objective To investigate the expression of miR - 34a and its target protein Foxpl in skin with psoriasis vulgaris and its significance in the development of psoriasis vulgaris. Methods The expressions of miR - 34a were studied with real-time fluorescent quantitative PCR in the 12 psoriatic vulgaris lesions and 12 normal skin. Chemical synthesis of miR - 34a mimic , miR - 34a inhibitor, transfected HaCaT cells. Western blotting was used to detect the expression of Foxpl protein in transfected cells. Results The expression level of miR - 34a was higher in lesions of psoriasis vulgaris than in normal skin tissue, and differences was statistical significance (t = 3. 047 ,P < 0. 05 ). HaCaT cells were transfected with miR - 34a mimic and miR - 34a inhibitor. Western blotting was used to detect the expression of Foxpl protein. When over expressing miR - 34a, the expression of Foxpl protein were increased. When miR-34a inhibited the expression of Foxpl, protein were decreased. And the differences was statistical significance( F = 286. 722 , P <0.01 ). Conclusion The expression of miR-34a was significantly higher in lesions of psoriasis vulgaris than in the normal skin tissue. Differential expression of miR-34a may be positive regulation of Foxpl protein in the pathogenesis of psoriasis vulgaris.