寻常痤疮患者肠道-宿主共代谢产物分析

Analysis of Gut Microbiota-host Co-metabolites in Patients with Acne Vulgaris

目的分析寻常痤疮患者与健康对照肠道-宿主共代谢产物差异,为肠道菌群参与寻常痤疮发病机制的研究提供理论依据。方法采集 43 例未经治疗的寻常痤疮患者和43例年龄、性别匹配的健康对照组的粪便标本,对入组者病程、身高、体重、睡眠质量等一般资料进行调查。采用 Agilent7890B型气相色谱仪对 118 种代谢产物进行定量分析,非参数检验比较两组代谢产物差异。结果寻常痤疮组与健康对照组代谢产物组成存在差异;不同病情严重程度痤疮患者代谢产物组成差异不明显;非参数检验分析发现24种肠道-宿主共代谢物含量在痤疮组和对照组之间存在明显的差异(P<0.05);另外,寻常痤疮患者睡眠质量较健康对照组差异有统计学意义(P<0.05);Pearson相关性分析提示肠道代谢产物与睡眠质量呈明显负相关。结论寻常痤疮患者与健康对照肠道-宿主共代谢产物差异明显,可能成为解释肠道菌群参与寻常痤疮发病机制的纽带。

Objective To analyze the differences of gut microbiota-host co-metabolites between patients with acne vulgaris and healthy controls,which could help the study of gut microbiota involved in the pathogenesis of acne vulgaris.Methods Fecal samples from 43 untreated acne patients and 43 age- and sex-matched healthy controls were collected.The disease duration,height,weight,and sleep quality of the participants were recorded.The 118 metabolites were detected using Agilent model 7890B gas chromatograph.Then the non-parametric tests were conducted to find the differences of gut metabolites between acne patients and healthy controls.Results Significant difference was found in the composition of metabolites between patients with acne vulgaris and healthy controls.There was no correlation of the metabolites with disease severity of the acne patients.Twenty-four different gut microbiota-host metabolites between patients and control group were found to be significantly different with non-parametric analysis(P<0.05).Compared with healthy controls,sleep quality was worse in patients with acne vulgaris(P<0.05).Pearson correlation analysis showed that the gut metabolites were negatively correlated with sleep quality.Conclusion Gut microbiota-host metabolites are significantly different between patients with acne vulgaris and healthy controls,which may be a link to explain the involvement of gut microbiota in the pathogenesis of acne vulgaris.