矮牵牛TPS家族基因的鉴定与PhTPS1的表达分析

Identification of TPS Family Genes and Expression Analysis of PhTPS1 in Petunia

研究表明海藻糖能够参与调控植物的分枝发育。本研究利用生物信息学方法,从矮牵牛全基因组数据库中鉴定出10个TPS家族成员基因。进化树分析显示,矮牵牛10个TPS蛋白家族成员分为ClassⅠ和ClassⅡ两大类。ClassⅠ中PhTPS1和PhTPS2均含有16个内含子。ClassⅡ中所有成员均含有2个内含子。TPS 10个成员中共找到6个Motif。PhTPS1、PhTPS2、PhTPS3、PhTPS4、PhTPS5、PhTPS8、PhTPS9、PhTPS10均含有Motif1~Motif6,PhTPS6和PhTPS7含有Motif1~Motif5。利用实时荧光定量PCR分析PhTPS1基因在不同组织、不同处理下的表达水平。组织特异性表达分析显示:PhTPS1在叶腋中最高,而花瓣中的表达量最低;对PhTPS1在去顶后及生长素和细胞分裂素处理后的表达水平进行了检测,结果表明:去顶6 h后PhTPS1显著上升,但随之逐渐下降;去顶后施加IAA则明显抑制了去顶引起的PhTPS1的上调;施加6-BA6 h能够引起PhTPS1表达水平的显著上升,但24 h后表达水平明显降低。该研究对于揭示矮牵牛分枝发育机理以及培育分枝特性不同的矮牵牛新品种均具有重要意义。

Studies have shown th at trehalose can participate in the regulation of plant shoot branching. In this study, ten TPS family genes were identified from the petunia genome database using bioinformatics methods.Phylogenetic tree analysis showed that 10 TPS protein family members of petunia were divided into Class Ⅰ and ClassⅡ. PhTPS1 and PhTPS2 in ClassⅠ both contained 16 introns. All members of ClassⅡ contained 2 introns.6 Motifs were found in the 10 TPS proteins. PhTPS1, PhTPS2, PhTPS3, PhTPS4, PhTPS5, PhTPS8, PhTPS9,PhTPS10 all contained Motif1~ Motif 6, while PhTPS6 and PhTPS7 contained Motif1~ Motif 5. Quantitative Real-time fluorescence PCR was used to analyze the expression level of PhTPS1 in different tissues and under different treatments. Tissue-specific expression analysis indicated that the expression of PhTPS1 was the highest in axillaes and the lowest in petals. The expression levels of PhTPS1 after decapitation or treatments with auxin and cytokinin were examined, the results of which showed that the expression of PhTPS1 increased significantly 6 h after decapitation, but then gradually decreased. The application of IAA after decapitation significantly inhibited the up-regulation of PhTPS1 caused by the decapitation;application of 6-BA for 6 h caused significant up-regulation of PhTPS1, but the expression levels decreased significantly after 24 h. This study could be of great significance for revealing the mechanism of shoot branching in Petunia hybrida and developing new cultivars with different branching characteristics.