拮抗菌HM-7培养基的优化

Optimized of the antagonistic bacteria HM-7

以拮抗菌HM-7的光密度值为评价指标,采用单因素试验和正交试验,对培养基中的碳源、氮源、无机盐进行了优化,利用分光光度法确定最优培养基,旨在提高拮抗菌HM-7的产菌量,为进一步研究拮抗菌HM-7提供一定的依据和参考,同时为生物防治在生活中广泛应用奠定基础。结果表明,拮抗菌HM-7的最佳培养基配方为葡萄糖2 g、酵母膏8 g、NaCl4g、蒸馏水1 000 mL、pH7.0,在此条件下培养24 h,更利于菌体生长,为扩大培养拮抗菌HM-7打下了基础。

The optical density of the antagonistic bacteria HM-7 was used as evaluation index, the carbon source nitrogen source and inorganic salt in the medium were optimized by single factor experiment and orthogonal experiment, and the optimal conditions were determined by spectrophotometry. The aim of this study was to improve the yield and to provide a basis for the further study of antagonistic bacteria HM-7, meanwhile, it would lay the foundation for the extensive appli cation of biological control in life. The results showed that the optimal medium of antagonistic bacteria HM-7 was glucose 2 g, yeast extract 8 g, NaCl4 g, distilled water 1000 ml, pH 7.0, under these conditions, the growth of bacteria was much better than other formulas. Our results laid a foundation for expanding the culture of antagonistic bacteria HM-7.