维管束发育相关基因sm-Nvas对水稻稻瘟病抗性分析

Analysis on Resistance of Tobacco Vascular Bundle Related Gene sm-Nvas to Rice Blast

[目的]探究烟草维管束发育相关基因sm-Nvas对水稻稻瘟病的抗性。[方法]构建pCAMBIA1305.1-sm-Nvas双元表达载体,利用农杆菌介导的愈伤转化法转化水稻YTB,对转基因阳性植株接种稻瘟病菌后进行抗性观察。[结果]成功构建了pCAMBIA1305.1-sm-Nvas双元超表达载体,获得了转sm-NvasYTB植株。离体接种稻瘟病菌后转基因植株的病斑比受体品种YTB的小。[结论]转sm-Nvas基因水稻具有稻瘟病抗性。

[Objective]To investigate the resistance of tobacco vascular bundle related gene sm-Nvas to rice blast.[Method]A plant binary expression vector pCAMBIA1305.1- sm-Nvas was constructed, and transformed to recipient variety YTB by Agrobacterium -mediated callus transformation. The resistance of the transgenic positive plant was investigated after inoculation with the rice blast fungi.[Result]The pCAMBIA1305.1- sm-Nvas binary over-expression vector was successfully constructed, and the transgenic YTB with sm-Nvas was obtained. The scab of the transgenic plant was smaller than that of the receptor variety YTB after inoculation of rice blast fungi in vitro .[Conclusion]The transgenic rice with sm-Nvas gene is resistance to rice blast.