CP-25通过调控T细胞亚群治疗大鼠胶原性关节炎

CP-25 alleviate collagen induced arthritis in rats by regulating T cell subsets

目的研究芍药苷-6-氧-苯磺酸酯(CP-25)对大鼠胶原诱导性关节炎(CIA)治疗作用及对T细胞亚群的影响。方法建立大鼠CIA模型,给予CP-25(50 mg/kg)治疗,艾拉莫德(10 mg/kg)为阳性对照药。测定足爪容积、肿胀关节数、关节和脾脏组织病理学和T细胞亚群等。结果 CP-25(50 mg/kg)可以显著降低CIA大鼠足爪体积和关节肿胀;减少脾脏白髓增生和滑膜组织炎性细胞浸润等;抑制T细胞增殖;减少CD3^+T细胞、CD3^+CD4^+T细胞、CD3^+ CD4^+ CD25^+ T细胞亚群,下调Th1、Th2和Th17细胞比率,且上述指标与阳性对照组差异无统计学意义。结论 CP-25对大鼠CIA有治疗作用,该作用可能与其调节活化T细胞亚群和分化T细胞亚群有关。

Objective To investigate the therapeutic effect of paeoniflorin-6’-O-benzenesulfonate(CP-25) on collagen induced-arthritis(CIA) in rats and regulation on T cell subsets. Methods Rats with CIA were established and administered with CP-25(50 mg/kg) in tragastric administration, and Iguratimod(10 mg/kg) was given as positive control drug. The therapeutic effects of CP-25 on the CIA of rats and its regulatory effects on the T cell subsets were evaluated by measuring the foot paw volume, the number of swollen joints, the histopathology of the joints and spleen and the T cell subgroup. Results CP-25(50 mg/kg) could significantly reduce the paw volume and joint swelling of CIA rats, reduce the white myelosis of spleen and synovial tissue inflammatory cell infiltration, inhibit the proliferation of T cells, reduce CD3^+ T cells, CD3^+ CD4^+ T cells, CD3^+ CD4^+ CD25^+ T cell subgroups, downregulate the ratio of Th1, Th2 and Th17 cells, and the above indicators were not statistically significant with the positive control group. Conclusion CP-25 has anti-inflammatory effects on rats with CIA, which may be related to regulating activated T cell subsets and the differentiation of T cell subsets.