FAM134B在肝癌组织中的表达及其与临床病理特征的关系

Expression of FAM134B in hepatocellular carcinoma and its relationship with clinicopathological features

目的检测42例肝癌组织中134序列相似的家庭成员B(FAM134B)的表达水平,结合临床病例资料分析FAM134B表达量与肝癌患者临床病理特征的关系。方法收集接受手术治疗的42例肝癌患者的组织标本及临床病例资料。标本包括切除的肝癌组织和配对的癌旁肝组织,均经本院病理科病理组织学确诊。分别采用免疫组化(IHC)、荧光定量聚合酶链反应(Real-time PCR)及免疫印迹(Western blot)检测FAM134B在肝癌组织和细胞中的表达量,并分析FAM134B表达量与肝癌患者临床病理特征的关系。结果免疫组化检测结果显示FAM134B在肝癌组织及癌旁组织阳性表达率分别为83%(35/42)和33%(14/42),差异有统计学意义(χ^2=12.343,P<0.05)。Real-time PCR检测结果显示FAM134B在肝癌组织中的mRNA表达高于癌旁组织(P<0.05)。并且FAM134B在HepG2细胞中的mRNA表达高于L02细胞(P<0.05)。Western blot检测结果显示:与癌旁组织比较,FAM134B在肝癌组织中高表达,与L02细胞相比,FAM134B在HepG2细胞中高表达。且FAM134B蛋白表达量与肿瘤大小、血清甲胎蛋白(AFP)、门静脉癌栓、肝外转移、邻近器官侵犯和分化程度显著相关性(P<0.05),即FAM134B表达量增高时,肿瘤更大、AFP更高、邻近器官侵犯更多、分化程度更低。然而与性别、年龄、乙肝、肝硬化、淋巴结侵犯无明显相关(P>0.05)。结论 FAM134B在肝癌中高表达,可能在肝癌的发生发展、侵袭转移中发挥作用。

Objective To investigate the expression of family with sequence similarity 134, member B(FAM134 B) in 42 cases of hepatocellular carcinoma and explore the relationship between FAM134 B expression and clinicopathological features of hepatocellular carcinoma.Methods A total of 42 paired specimens of tumor and adjacent non-tumor tissues and clinical data were collected from 42 cases of hepatocellular carcinoma patients and all the specimens were confirmed by histopathology. Immunohistochemistry(IHC), Real-time PCR and Western blot were used to examine the expression and distribution of FAM134 B in hepatocellular carcinoma tissues and cells. In addition, the correlation between the expression of FAM134 B and clinicopathological features of hepatocellular carcinoma was analyzed. Results IHC showed that the positive expression rate of FAM134 B in tumor tissues and adjacent non-tumor tissues were 83%(35/42) and 33%(14/42), the difference was statistically significant(χ^2=12.343,P<0.05). Real-time PCR showed that FAM134 B mRNA levels was significantly increased in liver tissues than in adjacent non-tumor tissues(P<0.05) and in HepG2 cells than in L02 cells(P<0.05). While Western blot showed that FAM134 B protein expression levels was higher in liver tissues compared with adjacent non-tumor tissues and higher in HepG2 cells compared with L02 cells. Moreover, the expression difference of FAM134 B was correlated with tumor size, serum alpha fetoprotein(AFP), portal vein tumor thrombosis, extrahepatic metastasis, adjaent organ invasion, differentiation grade(P<0.05), but not with gender, age, hepatitis B virus, cirrhosis and lymphatic invasion(P>0.05). In other words, when the expression level of FAM134 B increased, it had larger tumor size, higher AFP, deeper tumor adjacent tissues and lower defferentiation grade. Conclusion FAM134 B is highly expressed in hepatocellular carcinoma. And FAM134 B may plays an important role in the development, invasion and metastasis of hepatocellular carcinoma.