不同垫料对SPF级KM小鼠学习记忆能力的影响

Effect of Different Bedding on Learning and Memory Ability of Specific Pathogen-free KM Mice

目的探讨非专业加工制作的麦秸垫料、玉米芯垫料、刨花垫料和纸质垫料对SPF级昆明小鼠(KM)学习记忆能力的影响差异,以及不同垫料对小鼠行为学的影响。方法选用SPF级KM小鼠24只,分别使用麦秸垫料、刨花、玉米芯和纸质垫料饲养8周后,开展MORRIS水迷宫行为学实验,考察其行为学的变化和学习记忆能力的差异。结果麦秸组学习记忆能力增加明显高于刨花组(P<0.05);水迷宫实验期间,各组增重差异不显著。定位航行实验结果显示,麦秸组第5 d的逃避潜伏期显著低于其余三组(P<0.05),其余时间段差异无显著性;空间探索实验结果显示麦秸组小鼠穿过原平台位置的次数、游泳时间均多于其余三组。结论不同垫料对水迷宫实验中KM小鼠的体质量影响较小,麦秸垫料对KM小鼠学习记忆能力的影响优于刨花垫料和玉米芯垫料,建议将麦秸垫料作为实验动物接触性垫料推广使用。

Objective To investigate the differences of bedding materials consisted of wheat straw, wood shavings, corn and the paper on the body weight and the learning and memory ability of SPF KM mice. Method Twenty-four KM mice were feeding with wheat straw, wood shavings, corn and the paper beddings for 8 weeks. The MORRIS water maze behavior experiment was performed to determine the learning and memory skills and the body weight. Result Comparing with animals used the shavings bedding,the weight of the KM mice in the wheat straw bedding group was raised,significantly,P<0.05;The MWM result showed: the weight gain of each group was not significant. In the place navigation test, the wheat straw group was significantly lower than the other three groups(P<0.05) on the 5 th day;in the spatial probe test, KM mice used the wheat straw bedding spent more time in the target quadrant than other groups. Conclusion Different bedding material has little effect on body weight of KM mice in Morris water maze test,KM mice used the wheat straw bedding performed better than wood shavings and corn bedding in learning and memory ability, and the wheat straw bedding can be safely used in KM mice breeding.