维丙胺联合盐酸氟西汀对抑郁症小鼠的作用研究

The Effect Studying of Vinopropylamine Combined with Fluoxetine Hydrochloride on Depression Mouse Model

目的考察研究维丙胺胶囊联合盐酸氟西汀胶囊对小鼠抑郁症的作用效应。方法采用慢性应激加孤养的方法建立小鼠抑郁症模型。随机分为5组:空白对照组、模型组、维丙胺组、盐酸氟西汀组及维丙胺联合盐酸氟西汀组(联合用药组),每组10只,并连续30 d灌胃给药,观察各组小鼠的体质量变化、糖水偏爱率、强迫游泳不动时间及悬尾不动时间进行比较分析,综合评价联合用药对抑郁症小鼠的作用效果。结果建模完成时(给药第0天),各抑郁症模型组与空白对照比较,糖水总消耗量、糖水偏爱率及体质量均极显著性的降低(P<0.01)。灌胃给药后,各给药实验组动物体质量均有增长,第30天,与模型组比较,联合用药组体质量增长具有显著性差异(P<0.05),第39天,维丙胺组体质量增长具有显著性差异(P<0.05),第46天,联合给药组与盐酸氟西汀组比较,体质量增长具有显著性差异(P<0.05)。第50天,各给药实验组糖水总消耗量及糖水偏爱率均有不同程度升高,与模型组比较,维丙胺组糖水总消耗量及糖水偏爱率均具有显著性差异(P<0.05),与模型组比较,联合用药组糖水总消耗量具有显著性差异(P<0.05),联合用药组糖水偏爱率具有极显著性差异(P<0.01)。维丙胺组、联合用药组强迫游泳不动时间及悬尾不动时间显著减少,与模型组比较,维丙胺组游泳和悬尾不动时间均具有显著性差异(P<0.05),联合用药组悬尾不动时间具有极显著性差异(P<0.01),与盐酸氟西汀组比较,联合用药组悬尾不动时间具有显著性差异(P<0.05)。结论通过实验提示维丙胺具有抗抑郁作用,且联合盐酸氟西汀效果更佳,本研究将为临床抑郁症的临床用药提供实验依据与参考。

Objective To investigate the effect of vinopropylamine capsules combined with fluoxetine hydrochloride on depression mice;Method The depression model was established by the method of chronic stress and isolation;Randomly divided roup and vinopropyla mine combined with fluoxetine hydrochloride group(combination drug group), each group ten mice and were administered drug by gavage for 30 days, the weight change, sugar water preference rate, the fixed time of forced swimming and suspended tail, and were compared and analyzed, and was comprehensively evaluated the effect of the combined drug on depression mice;Result When the moding was established(the 0 th day of administration), the total consumption of sugar water, the preference rate of sugar water and the weight of mice in each group were extremely significant reduction(P<0.01);After intragastric administration, the weight of mice were increased in each experimental group, on the 30 th day, compared with the model group, the combined treatment group was significant(P<0.05), on the 39 th day, the vinopropylamine group was significant(P<0.05), and compared with the fluoxetine hydrochloride group on the 46 th day, the ponderal growth of combined treatment group was significant(P<0.05);On the 50 th day, each dose group of sugar water consumption and sugar preference rate were different extent increased, compared with model group, the sugar water consumption and sugar preference rate of vinopropylamine group were significant(P<0.05), and the combination drug group of sugar water consumption had significance(P<0.05), the sugar water preferred rate had a extremely significant difference(P<0.01);The immobility time of forced swimming and suspension tail were significantly reduced of the vinopropylamine group and the combined drug group, compared with the model group, the immobility time of forced swimming and suspension tail of the vinopropylamine group were significant(P<0.05), the immobility time of suspension tail of the combined drug group had extremely significant differences(P<0.01), and the immobility time of the suspension tail of the combined drug group had significance(P<0.05) compared with the fluoxetine hydrochloride group;Conclusion The results of this studying suggest the vinopropylamine had antidepressant effect and combined fluoxetine hydrochloride was more effective, this studying will provide experimental basis and reference for clinical depression combination drug.