摘要
抗菌肽是一类具有广谱抑菌活性功能的特殊生物活性肽,在医药、食品、农业等领域都有广泛的应用。本文总结了抗菌粗肽预处理方法的适用范围及特点,并重点综述了抗菌肽的快速筛选方法,包括抗菌肽-细菌吸附结合法、细胞膜色谱法、模拟细胞膜色谱法、毛细管电泳法、薄层色谱自显影法和高通量测序法等,为发掘抗菌肽、丰富抗菌肽库提供一定的参考。
Antimicrobial peptides are a class of special bioactive peptides with broad-spectrum antibacterial activities, and are extensively applied in medicine, food, agriculture and other fields. This paper summarizes the applicability and characteristics of various pretreatment methods for detection of antibacterial peptides in crude samples. Emphasis is placed on the rapid screening methods for antimicrobial peptides, including bacterial adsorption method, cell membrane af finity chromatography, simulated cell membrane af finity chromatography, capillary electrophoresis, thin layer chromatography autoradiography and high throughput sequencing. This review will provide useful information for discovering new antimicrobial peptides and enriching the existing bank of antimicrobial peptides.
作者
薛辉
涂勇刚
熊春红
李建科
罗文翔
赵燕
XUE Hui;TU Yonggang;XIONG Chunhong;LI Jianke;LUO Wenxiang;ZHAO Yan(Engineering Research Center of Biomass Conversion,Ministry of Education,State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China;College of Food Science and Engineering,Jiangxi Agricultural University,Nanchang 330045,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2019年第13期334-339,共6页
Food Science
基金
江西省杰出青年基金项目(2018ACB21031)
江西省青年科学家培养对象计划项目(20153BCB23028)
江西省杰出青年人才资助计划项目(20162BCB23031)
江西省科技支撑计划项目(20151BBF60022)
江西省自然科学基金项目(20132BAB214008)
南昌大学研究生创新专项基金项目(CX2018123)
关键词
抗菌肽
抑菌
快速筛选
制备
antimicrobial peptides
antibacterial
rapid screening
preparation
