八珍汤对荷结肠癌小鼠皮下移植瘤中CXCR4和CD133共表达的影响

Effect of Bazhen Decoction on co-expression of CXCR4 and CD133 in subcutaneously transplanted tumor of mice with colon cancer

目的观察八珍汤对结肠癌皮下移植瘤模型小鼠癌组织中CXCR4及CD133共表达的影响,探讨八珍汤抑制结肠癌移植瘤生长的机制。方法将70只裸鼠随机分为模型组35只、八珍汤组35只。2组均采用结肠癌CT26细胞混悬液直接注射的方法建立皮下移植瘤模型,造模后第10天起,模型组以无菌蒸馏水灌胃,八珍汤组以八珍汤灌胃,均连续灌胃10d。自灌胃开始后第2,4,6,8,10天测量肿瘤大小并计算体积,然后计算抑瘤率。灌胃后的第11天处死2组裸鼠,取瘤组织,常规HE染色观察,并采用免疫组化法检测瘤组织中CXCR4和CD133表达情况。结果灌胃后第2,4,6,8,10天,八珍汤组肿瘤体积均明显小于模型组(P均<0.05),八珍汤组抑瘤率为56.13%。移植瘤组织HE染色显示,八珍汤组较模型组细胞异型性小,且细胞排列成团现象不明显。2组CXCR4、CD133蛋白阳性表达率比较虽然差异均无统计学意义(P均>0.05),但八珍汤组约较模型组降低10%。结论八珍汤可以通过抑制CXCR4及CD133的共表达来抑制肿瘤的生长。

Objective It is to observe the effect of Bazhen Decoction on the co-expression of CXCR4 and CD133 in mouse cancer tissues of colon cancer subcutaneous xenograft model,and to explore the mechanism of Bazhen Decoction in inhibiting the growth of colon cancer xenografts.Methods 70 nude mice were randomly divided into model group ( n =35) and Bazhen decoction group ( n =35).The subcutaneous xenograft model was established by direct injection of colon cancer CT26 cell suspension in the two groups.From the 10th day after model establishment,the model group was perfused with sterile distilled water,and the Bazhen Decoction group was administered with Bazhen Decoction,both groups were treated continuously by gavage for 10d.The tumor size was measured and volume was calculated on days 2,4,6,8,and 10 after the start of gavage,and then the tumor inhibition rate was calculated.On the 11th day after intragastric administration,the mude mice in the two groups were sacrificed.The tumor tissues were taken and observed by routine HE staining.The expression of CXCR4 and CD133 in tumor tissues was detected by immunohistochemistry.Results On the 2nd,4th,6th,8th and 10th day after intragastric administration,the tumor volume of Bazhen Decoction group was significantly lower than that of the model group ( P <0.05),and the inhibition rate of Bazhen Decoction group was 56.13%.HE staining of transplanted tumor tissue showed that the Bazhen decoction group had smaller cell type than the model group,and the cell arrangement was not obvious.There was no significant difference in the positive expression rates of CXCR4 and CD133 between the two groups ( P >0.05),but the Bazheng group was reduced by 10% compared with the model group.Conclusion Bazhen Decoction can inhibit tumor growth by inhibiting the co-expression of CXCR4 and CD133.