小檗碱对A549细胞增殖、迁移和侵袭的影响

Effects of berberine on the proliferation, migration and invasion of A549 cells

目的研究小檗碱对肺癌细胞A549增殖、迁移和侵袭的影响。方法培养THP-1细胞并加入小檗碱后,MTT法检测细胞增殖。100 ng/mL PMA和20 ng/mL IL-4将THP-1细胞诱导成M2型肿瘤相关巨噬细胞,小檗碱预给药后,随机分为对照组、模型组、小檗碱组,细胞培养上清与A549细胞共培养。RT-PCR、Western blot、ELISA检测CD206、IL-10、TGF-β表达,Transwell检测细胞迁移和侵袭。结果小檗碱最佳刺激浓度为20μmol/L。与模型组比较,小檗碱组可显著抑制CD206、IL-10、TGF-β表达(P<0.05)。小檗碱可抑制THP-1细胞转化为M2型肿瘤相关巨噬细胞。结论小檗碱可调节A549细胞的增殖、侵袭和迁移,其机制可能与抑制肿瘤相关巨噬细胞向M2型巨噬细胞的转化有关。

AIM To study the effects of berberine on the proliferation, migration and invasion of lung cancer A549 cells.METHODS Berberine was added with cultured THP-1 cells, after which cell proliferation was examined by MTT assay. THP-1 cells induced into M2 tumor-associated macrophages with 100 ng/mL PMA and 20 ng/mL IL-4 were then pre-administered with berberine and divided into control group, model group and berberine group, cell culture supernatant was co-cultured with A549 cells. The expressions of CD206, IL-10 and TGF-β were detected by RT-PCR, Western blot and ELISA, cell migration and invasion were detected by Transwell.RESULTS The optimal stimulation concentration of berberine was 20 μmol/L. Compared with the model group, the berberine group demonstrated significantly up-regulated expressions of M2 markers CD206, IL-10 and TGF-β(P<0.05). Berberine inhibited the transformation of THP-1 cells into M2 type tumor-associated macrophages.CONCLUSION Berberine can regulate the proliferation, invasion and migration of A549 cells, whose mechanisms may contribute to the inhibition of transformation of tumor-associated macrophages into M2 macrophages.