超声造影剂SonoVue经皮穿刺注入兔肝组织超声表现的实验研究

Experimental study of ultrasound contrast agent SonoVue injected into rabbit liver tissue by percutaneous puncture

目的观察SonoVue在经皮注入兔肝组织后的弥散规律、超声变化特点及生物学效应。方法30只新西兰健康大白兔分为注射造影剂0.1mL组、0.2mL组、0.3mL组、0.4mL组、0.5mL组和注射生理盐水的对照组。各实验组经皮穿刺兔肝组织注射相应剂量造影剂,对照组注射生理盐水。观察造影剂消退情况。造影剂消退后处死兔子,取造影区肝组织行HE染色,观察肝组织损伤情况。结果注射区平均灰度值较周围肝组织明显增高,随剂量增加平均灰度值增高,超声造影剂代谢缓慢,造影时间显著延长。重复测量资料方差分析显示,不同注射组不同时间点平均灰度值变化值差异具有统计学意义(P=0.000)。实时超声动态观察发现动静脉灌注增强现象、造影增强区边缘钝化现象、造影区繁星征。病理结果显示,肝细胞形态正常,未见肿胀变形,周围组织结构排列整齐。肝组织间质内见淋巴细胞聚集。结论超声造影剂经皮穿刺注入兔肝组织增强时间显著延长,造影安全可行。

Objective To observe the diffusion rule, ultrasound changes and biological effects of ultrasound contrast agent SonoVue after percutaneous injection into rabbit liver tissue. Methods Thirty New Zealand healthy white rabbits were divided into experimental group and control group. Each experimental group was further divided into five subgroups according to the dose of contrast injection percutaneous injection, which were 0.1 mL group, 0.2 mL group, 0.3 mL group, 0.4 mL group, and 0.5 mL group. The control group was injected with the same dose of physiological saline. Dynamic observation of contrast agent regression in the injection area. After the contrast agent was completely ablated, the rabbit was sacrificed, and the liver tissue of the contrast area was taken for hematoxylin-eosin staining to observe the liver tissue damage. Results The average gray value of the injected contrast agent area was significantly higher than that of the surrounding liver tissue. The average gray value of the injection area increased, the duration of angiography was proportional to the dose increase, and inversely proportional to the metabolic rate of ultrasound contrast agent. The interaction between groups, time points, and time between groups were statistically significant( P <0.05). The observation of real-time dynamic ultrasound revealed enhanced phenomenon of arteriovenous perfusion, edge passivation in contrast enhanced area, and star-shaped sign in contrast area. The pathological results showed that the liver cells in the injection area were normal, no swelling and deformation, the surrounding tissue structure was neatly arranged, and lymphocytes were found in the interstitial tissue of the liver tissue. Conclusion Percutaneous injection of ultrasound contrast agent can significantly prolong the enhancement time of rabbit liver tissue, and percutaneous injection of contrast agent is safe and feasible.