淫羊藿苷干预磷酸钙骨水泥/骨髓间充质干细胞复合体修复兔股骨缺损

Effect of icariin on the repair of femoral defects in rabbits by calcium phosphate bone cement/bone marrow mesenchymal stem cell complex

背景:已有研究显示磷酸钙骨水泥与骨髓间充质干细胞复合物有助于修复软骨缺损。淫羊藿苷是一类黄酮类物质,其对细胞增殖和分化有明显促进作用。目的:观察淫羊藿苷干预下磷酸钙骨水泥/骨髓间充质干细胞复合体修复兔股骨缺损的效果。方法:取30只新西兰大白兔(海南医学院实验动物中心提供),随机分为3组并制作右侧股骨末端骨缺损模型:空白组股骨缺损不填充材料,对照组骨缺损处植入成骨诱导培养的骨髓间充质干细胞与磷酸钙骨水泥复合物,实验组股骨缺损处植入淫羊藿苷干预且经成骨诱导培养的骨髓间充质干细胞与磷酸钙骨水泥复合物。术后4,8,12周进行骨缺损部位影像学、生物力学分析。动物实验获得海南医学院第一附属医院伦理委员会批准。结果与结论:①术后4,8,12周Micro-CT显示,实验组成骨速度明显优于空白组和对照组;术后第12周,对照组中磷酸钙骨水泥有部分被新骨代替,骨痂未完全包绕,实验组股骨缺损区已完全有新骨生成,植入的磷酸钙骨水泥已完全降解;实验组和对照组术后12周的骨小梁模式因子、骨体积分数、骨小梁数量均明显高于空白组(P<0.05),并且实验组各项指标优于对照组(P<0.05);②实验组术后4,8,12周的骨密度高于对照组、空白组(P<0.05);③实验组术后12周的骨标本抗弯曲能力的最大应力、最大负荷力和破坏能量明显高于对照组、空白组(P<0.05);④结果表明,淫羊藿苷干预后的骨髓间充质干细胞/磷酸钙骨水泥复合物能有效促进新骨形成,加快骨修复。

BACKGROUND: Previous studies have shown that calcium phosphate bone cement/bone marrow mesenchymal stem cell complex can help repair cartilage defects. Icariin is a kind of flavonoids that can promote cell proliferation and differentiation. OBJECTIVE: To investigate the effect of icariin-treated calcium phosphate cement/bone marrow mesenchymal stem cell complex in the repair of femoral defects in rabbits. METHODS: Thirty New Zealand white rabbits were provided by Laboratory Animal Center, Hainan Medical University, China and prepared into models of right femur end defect. Then these models were randomly divided into three groups. In the blank group, there was no filling material for femoral defect. In the control group, the complex of calcium phosphate bone cement and the bone marrow mesenchymal stem cells which were cultured from osteoblasts was implanted in the femoral defect area. In the experimental group, the complex of calcium phosphate bone cement and the bone marrow mesenchymal stem cells which were cultured from osteoblasts and treated with icariin was implanted in the femoral defect area. Imaging and biomechanical analysis of bone defects were performed at 4, 8 and 12 weeks after surgery. Animal experiments were approved by the Ethics Committee, First Affiliated Hospital of Hainan Medical University, China. RESULTS AND CONCLUSION: At postoperative 4, 8 and 12 weeks, micro-CT scans showed that the osteogenesis rate of the experimental group was significantly faster than that of the blank and control groups. At 12 weeks after surgery, some calcium phosphate bone cement was replaced by new bone, and callus was not completely enclosed in the control group. New bone formed in the femoral defect area, and the implanted calcium phosphate bone cement was completely degraded in the experimental group. At 12 weeks after surgery, trabecular mode factor, bone volume fraction, and the number of trabeculae in the experimental and control groups were significantly greater than those in the blank group (P < 0.05). The indexes in the experimental group were better than those in the control group (P < 0.05). At 4, 8 and 12 weeks after surgery, bone density in the experimental group was significantly higher than that in the control and blank group (P < 0.05). At 12 weeks after surgery, the maximum stress, maximum load and destructive energy in the experimental group were significantly greater than those in control and blank groups (P < 0.05). These results suggest that icariin-treated bone marrow mesenchymal stem cells/calcium phosphate bone cement complex can effectively promote new bone formation and accelerate bone repair.