尿液中肌氨酸、PCA3mRNA在前列腺癌早期诊断中的应用

Application of Urinary Sarcosine and PCA3mRNA in Early Diagnosis of Prostate Cancer

目的探讨尿液中肌氨酸、PCA3mRNA,血清PSA在前列腺癌早期临床诊断中的应用。方法收集2016年9月~2017年12月在我院收治的前列腺增生患者62例以及前列腺癌患者62例(病理确诊)作为研究对象,前列腺癌患者为研究组,前列腺增生患者为对照组。以两组患者进行前列腺按摩后的初始尿液为标本,采用实时荧光定量对肌氨酸、PCA3mRNA,血清PSA进行检测,比较两组患者肌氨酸、PCA3mRNA,血清PSA的差异并统计两组患者的诊断准确度。结果研究组患者诊断准确度高于对照组,差异具有统计学意义(P<0.05);研究组患者尿肌氨酸、PCA3mRNA,血清PSA水平分别为(13.51±2.54)ng/ml、(6.09±0.56)ng/ml、(0.53±0.07)μmol/L,高于对照组的(7.60±0.27)ng/ml、(2.03±0.21)ng/ml、(0.36±0.04)μmol/L,差异具有统计学意义(P<0.05)。结论早期联合监测尿液中的肌氨酸、PCA3mRNA及血清PSA水平,可以提高前列腺癌的监测有效率,为患者提供更为便利的诊断手段。

Objective To investigate the application of sarcosine,PCA3mRNA and serum PSA in the early diagnosis of prostate cancer.Methods A total of 62 patients with benign prostatic hyperplasia and 62 patients with prostate cancer (pathological diagnosis) were enrolled in our hospital from September 2016 to December 2017.The patients with prostate cancer were the study group and the patients with benign prostatic hyperplasia were the control group.The initial urine of the two groups of patients after prostate massage was used as the specimen.Real-time fluorescence quantification was used to detect sarcosine,PCA3mRNA and serum PSA.The differences of serum PSA between serum and PCA3mRNA were compared and the diagnostic accuracy of the two groups were statistically analyzed.Results The diagnostic accuracy of the study group was higher than that of the control group,the difference was statistically significant (P<0.05).The urinary creatinine,PCA3mRNA and serum PSA levels in the study group were (13.51±2.54) ng/ml,(6.09± 0.56) ng/ml,(0.53±0.07)μmol/L,higher than the control group (7.60±0.27) ng/ml,(2.03±0.21) ng/ml,(0.36±0.04)μmol/L,the difference was statistically significant (P<0.05).Conclusion Early monitoring of urinary creatinine,PCA3mRNA and serum PSA levels in urine can improve the efficiency of prostate cancer monitoring and provide a more convenient diagnostic tool for patients.