MMP-9抑制剂对口腔鳞癌细胞增殖和侵袭能力的影响

Effect of MMP-9 inhibitor on oral squamous cell carcinoma

目的 观察MMP-9抑制剂对人口腔鳞癌细胞系CAL27增殖和侵袭能力的影响。方法 选取人口腔鳞癌细胞系CAL27进行培养,用不同浓度的MMP-9抑制剂BB-94处理对数期CAL27细胞,通过RT-PCR检测BB-94在基因水平上对MMP-9的抑制效果。细胞免疫化学实验检测BB-94在蛋白水平上对MMP-9的抑制效果。MTT实验检测MMP-9抑制剂BB-94对人口腔鳞癌细胞增殖能力的影响。划痕实验检测MMP-9抑制剂BB-94对人口腔鳞癌细胞迁移能力的影响;Transwell实验检测MMP-9抑制剂BB-94对人口腔鳞癌细胞侵袭能力的影响。结果 MMP-9抑制剂能有效抑制人口腔鳞癌细胞CAL27的增殖、迁移和侵袭能力。结论 抑制MMP-9的表达可以抑制人口腔鳞癌细胞CAL27的增殖、迁移和侵袭等恶性生物学行为。

Objective To observe the effect of MMP-9 inhibitor on human oral squamous cell carcinoma (OSCC) cell line CAL27. Methods OSCC cell line CAL27 was cultured in DMEM high glucose medium with 10% FBS and the logarithmic phase CAL27 cells were treated with different concentrations of MMP-9 inhibitor BB-94. The inhibiting effect of BB-94 on MMP-9 in the gene level was detected by RT-PCR assay. The inhibiting effect of BB-94 on MMP-9 in the protein level was detected by cell immunochemistry assay. The effect of BB-94 on the proliferation of human oral squamous cell carcinoma cells was detected by MTT assay. The effect of BB-94 on the migration ability of OSCC was examined by scratching assay. Transwell experiment examined the invasion ability of human oral squamous cell carcinoma. Results MMP-9 inhibitor could effectively inhibit the proliferation, migration and invasion of OSCC cells CAL27. Conclusion Inhibiting the expression of MMP-9 has a significant inhibitory effect on the malignant biological behavior of OSCC cells CAL27.