原花青素对慢性不可预知性应激小鼠脑内单胺递质的影响

Effect of Proanthocyanidin on Monoamine Expression of Mice with Unpredictable Chronic Mild Stress

目的研究原花青素在慢性不可预知性应激(unpredictable chronic mild stress,UCMS)小鼠中的抗抑郁样作用以及对小鼠脑内单胺递质含量的影响。方法随机将ICR 小鼠分为6 组:空白对照组,UCMS 组,原花青素低、中、高剂量组(UCMS+原花青素12.5,25,50 mg·kg^-1),氟西汀组(UCMS+氟西汀10 mg·kg^-1)。采用UCMS 建立模型组评估小鼠的抑郁和焦虑样行为,采用HPLC 测定小鼠海马、额叶皮层和下丘脑中单胺递质的含量。结果与空白对照组比较,UCMS组小鼠悬尾的不动时间明显增加(P<0.05),大理石掩埋数目显著增加(P<0.01);小鼠海马、额叶皮层和下丘脑中的去甲肾上腺素、5-羟色胺和多巴胺的含量均减少;与UCMS 组比较,原花青素组能明显逆转上述行为学以及脑内单胺递质含量的改变。结论 UCMS 小鼠存在抑郁焦虑样行为学的改变,海马、额叶皮层和下丘脑中单胺递质含量表达异常。原花青素可以改善UCMS 小鼠行为学的改变,并调节海马、额叶皮层和下丘脑中单胺递质的表达。

OBJECTIVE To explore the effect of proanthocyanidin on unpredictable chronic mild stress(UCMS) mice with depression-like behavior,and its effect on the content of monoamine.METHODS ICR mice were divided into 6 groups: blank group,UCMS group,proanthocyanidin low,medium,high dose groups(UCMS+proanthocyanidin 12.5,25 and 50 mg·kg^-1), fluoxetine group(UCMS+fluoxetine 10 mg·kg^-1).The model of mice was established by UCMS,and then to evaluate depression-like behavior and anxiety-like behavior.The content of monoamine in the hippocampus,cortex and hypothalamus was determined by HPLC.RESULTS Compared with the control group,the duration of immobility of UCMS mice in the tail suspension test significantly increased(P<0.05),the number of the buried marble of UCMS mice increased(P<0.01).The expression of norepinephrine(NE),5-hydroxytryptamine(5-HT) and dopamine(DA) decreased in the hippocampus,prefrontal cortex and hypothalamus of UCMS model group as compared with those in the control group.Proanthocyanidin improved the behavior of UCMS mice,and reversed the levels of 5-HT,NE and DA in the hippocampus,prefrontal cortex and hypothalamus. CONCLUSION UCMS model mice have abnormal change in the depression and anxiety-like behavior and expression of monoamine in the hippocampus,prefrontal cortex and hypothalamus.Proanthocyanidin can ameliorate the changes of the behavior and regulate the expression of monoamine in the hippocampus,prefrontal cortex and hypothalamus.