巨噬细胞加帽蛋白的表达对前列腺癌细胞增殖和迁移能力的影响

Effect of macrophage capping protein expression on proliferation and migration of prostate cancer cells

目的观察巨噬细胞加帽蛋白(CapG)在前列腺癌中的表达及其生物学功能.方法采用免疫组织化学检测2013年1月至2018年6月间就诊于温州医科大学附属第二医院的115例前列腺癌(PCa)和42例邻近正常前列腺的CapG表达,通过实时定量反转录聚合酶链反应(RT-qPCR)和蛋白质印迹法(Westernblot)检测3例前列腺癌组织细胞中CapG的表达.利用小干扰RNA(siRNA)特异性敲低CapG表达,通过噻唑蓝(MTT)、Transwell小室实验检测CapG对人前列腺癌细胞增殖和迁移的影响.应用SPSS17.0统计软件分析,采用Pearson的χ^2检验评估CapG表达与临床病理学参数之间的关系.用独立样本t检验来比较CapG蛋白高表达组和低表达组间差异.结果前列腺癌和邻近正常前列腺组织中CapG表达的阳性率分别为55.6%和16.7%,差异有统计学意义(χ^2=18.879,P<0.05);CapG表达与前列腺癌的前列腺特异抗原(PSA)水平、Gleason评分、T分期和淋巴结转移显著相关(P<0.05).下调CapG后明显抑制PC3、DU145细胞的增殖和迁移能力(P<0.05).结论CapG在前列腺癌中高表达,并影响前列腺癌细胞的增殖和迁移能力.

Objective To investigate the expression and explore the biological function of macro phage capping protein ( CapG) in prostate cancer ( PCa). Methods Immunohistochemistry was used to detect the expression of CapG in 115 cases of prostate cancer and 42 cases of adjacent normal prostate in the Second Affiliated Hospital of Wenzhou Medical University from January 2013 to June 2018. The correlation between CapG expression and clinicopathological features in PCa was evaluated by statistical methods. The expression of CapG in 3 prostate cancer tissues was detected by real-time quantitative reverse transcriptase-polymerase chain reaction ( RT-qPCR) and Western blotting. CapG expression was knocked down by small interfering RNA ( siRNA), and the effect of CapG on proliferation and migration of human prostate cancer cells was detected by methyl thiazol tetrazolium ( MTT)(5 g/L) and Transwell chamber experiments. Results The positive rates of CapG expression in prostate cancer and adjacent normal prostate tissues were 55. 6% and 16. 7%, respectively, and the difference was statistically significant (χ^2 = 18. 879, P < 0. 05 );CapG expression was significantly correlated with prostate specific antigen (PSA) level, Gleason score, T stage and lymph node metastasis of prostate cancer ( P < 0. 05 ). Down-regulation of CapG significantly inhibited the proliferation and migration of PC3 and DU145 cells (P < 0. 05 ). Conclusion CapG is highly expressed in prostate cancer and affects the proliferation and migration of prostate cancer cells.