长链非编码RNA-SAP30L-AS1在前列腺癌组织的表达及临床意义

Expression and clinical significance of long-chain non-coding RNA-SAP30L-AS1 in prostate cancer

目的探讨长链非编码RNA-SAP30L-AS1在前列腺癌组织的表达及临床意义.方法术后病理证实前列腺癌组织标本76例,其中前列腺直径≥2.5cm39例,<2.5cm37例,低危组38例,高危组38例,应用实时定量反转录聚合酶链反应(RT-qPCR)检测76例患者前列腺癌组织中的长链非编码RNA-SAP30L-AS1的表达,应用SPSS21.0统计软件分析,计数资料采用x^2检验,并使用Pearsonkx^2连续校正分析长链非编码RNA-SAP30L-AS1在低危及高危前列腺癌组织的表达差异.结果长链非编码RNA-SAP30L-AS1的表达量与肿瘤高分级、临床分期及淋巴结转移明显相关(Gleason评分,χ^2=4.828,P<0.05;临床分期,χ^2=8.901,P<0.01;淋巴结转移阳性,χ^2=4.070,P<0.05),而与年龄及肿瘤大小表达无明显相关(P>0.05).结论长链非编码RNA-SAP30L-AS1在肿瘤高分级及临床高分期中高表达,其在前列腺癌发生发展中具有重要作用.

Objective To study the expression and clinical significance of long-chain non-coding RNA-SAP30L-AS1 in prostate cancer.Methods 76 prostate cancer specimens were confirmed by pathology after operation,including 39 cases with prostate diameter ≥2.5 cm,<2.5 cm in 37 cases,38 cases in low-risk group and 38 cases in high-risk group.The expression of long-chain non-coding RNA-SAP30L-AS1 in 76 patients with prostate cancer was detected by real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR).The difference of expression of long-chain non-coding RNA-SAP30L-AS1 in low-risk and high-risk prostate cancer tissues was analyzed statistically.Results Long-chain non-coding RNA-SAP30L-AS1 was associated with high grade,clinical stage and lymph node metastasis (Gleason score,χ2 =4.828,P < 0.05;clinical stage,χ2 =8.901,P <0.01;the positive of lymph node metastasis,χ2 =4.070,P < 0.05),but in age and tumor size and was not significantly (P > 0.05).Conclusion Long-chain non-coding RNA-SAP30L-AS1 is highly expressed in high grade and clinical stages,which indicates that it plays an important role in the occurrence and development of prostate cancer.