超声提取/气相色谱法检测食用槟榔中的甜蜜素

Detection of Cyclamate in Edible Areca Nut by Ultrasonic Extraction/gas Chromatography

建立了使用超声提取/气相色谱测定槟榔中甜蜜素的方法,以甜蜜素提取率为指标,采用单因素试验和正交试验的方法考查提取温度、超声时间、料液比对槟榔中甜蜜素提取的影响。结果表明,提取温度60℃,超声时间60min,料液比1∶15时槟榔中提取所得甜蜜素含量最高。在优化条件下,该方法的线性范围在0.2~1.8mg/mL,R2=0.9991,检测限为13.99μg/mL,在高、中、低3个加标水平下的回收率在98.0%~100.6%,RSD为2.62%(n=6)。该方法快捷准确、灵敏度好,具有良好的重复性和分离度,可以有效避免样品中各种成分的干扰,适合检测食用槟榔中甜蜜素的含量。

To establish a method of ultrasonic extraction/gas chromatography for sodium cyclamate in betel nut,the effect of the extraction temperature,ultrasonic action time,liquid-solid ratio of the sodium cyclamate in betel nut by single-factor and orthogonal experiment based on extraction ratio. The results showed that the optimal conditions were extraction temperature 60 ℃,ultrasonic action time 60 min,solid-liquid ratio 1∶15. Under this conditions,linear range 0.2~1.8 mg/mL,R2= 0.999 1,limit of detection(LOD)13.99 μg/mL,the average recoveries at three spiked levels of high,middle,low range from 98.0%~100.6%,RSD was 2.62%(n=6). The method was rapider,accurate,sensitive,reproducible,resolving power was good,effectively avoid distraction from other component and suitable for determinationofsodium cyclamate in betel nut.