基于多指标评价优选草珊瑚炮制工艺

Optimization of processing technology of Sarcandra glabra based on multi-index evaluation method

目的运用层次分析法(AHP)建立一种中药多指标的综合评价方法,优选草珊瑚最佳炮制工艺。方法在单因素实验基础上,通过AHP以新绿原酸、绿原酸、隐绿原酸、咖啡酸、异嗪皮啶、落新妇苷、迷迭香酸含量的综合评分为评价指标,以烘干温度、烘干时间为影响因素,通过单因素考察确定草珊瑚叶的净制工艺;以浸泡时间、闷润时间、切制长度、烘干温度为影响因素,通过星点设计-效应面法优选草珊瑚茎的最佳切制工艺;利用多指标AHP对优选工艺进行分析与验证。结果草珊瑚茎最佳炮制工艺为茎抢水洗净1次,12倍量水浸泡1 h,闷润软化3 h,取出后切制成15 mm的段,于50℃烘2 h;叶的炮制方法为叶抢水清洗1次,置50℃干燥4 h;再将炮制好的草珊瑚茎与叶2:1混合。最优的草珊瑚茎软化切制方法的综合评分的验证值与预测值的偏差为4.70%。结论优化的炮制工艺简单、稳定、可行,所得参数可为草珊瑚的研究生产提供科学依据,同时AHP为中药多指标评价研究提供借鉴。

Objective Analytic hierarchy process (AHP) was used to establish a comprehensive evaluation method of multiple indexes of traditional Chinese medicine, and the best processing technology of Sarcandra glabra was optimized. Methods On the basis of single factor experiment, the comprehensive score of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, isofraxidin, astilbin and rosmarinic acid content was used as evaluation index by AHP, and drying temperature and drying time were used as influencing factors. The purification process of S. glabra leaves was determined by single factor investigation. Taking soaking time, soaking time, cutting length and drying temperature as influencing factors, the optimal cutting process of S. glabra stems was optimized by central composite design-response surface method. Multi-index analytic hierarchy process (AHP) was used to analyze and verify the optimal process. Results The best processing technology of S. glabra stems was as follows:stem Rob water washed once, soaked in 12 times of water for 1 h, moistened and softened for 3 h, cut into 15 mm segments after taking out, and baked at 50℃ for 2 h;The processing method of leaves was as follows:leaf Rob water washed once and dried at 50℃ for 4 h;Then mixed the processed stems and leaves of S. glabra at 2:1. The deviation between the verification value and the prediction value of the comprehensive score of the optimal S. glabra stem by softening and cutting method was 4.70%. Conclusion The processing technology is simple, stable and feasible. The obtained parameters can provide scientific basis for the research and production of S. glabra, and AHP can provide reference for the multi-index evaluation of traditional Chinese medicine.