敲低白细胞介素20受体2(IL-20R2)抑制咪喹莫特诱导的小鼠银屑病发生

Knockdown of interleukin 20 receptor 2 (IL-20R2) inhibits the development of psoriasis induced by imiquimod in mice

目的观察敲除白细胞介素20受体2(IL-20R2)对咪喹莫特致小鼠银屑病的影响。方法采用IL-20R2基因敲除(IL-20R2^-/-)小鼠、野生型IL-20R2^+/+小鼠, PCR检测小鼠皮肤组织IL-20R2 mRNA水平, Western blot法检测IL-20R2蛋白水平。在各种小鼠背部裸露皮肤连续涂抹咪喹莫特7 d,每日观察拍照并记录小鼠体质量变化,取背部皮肤进行HE染色观察皮肤的病变情况。结果与IL-20R2^+/+小鼠相比, IL-20R2^-/-小鼠皮肤组织IL-20R2mRNA和蛋白水平降低,这些小鼠涂抹咪喹莫特后几乎不出现银屑病样症状,而野生型小鼠随着涂抹咪喹莫特天数的增加银屑病样症状不断加重。结论敲低IL-20R2可抑制咪喹莫特诱导的小鼠银屑病的发生。

Objective To investigate the role of interleukin 20 receptor 2(IL-20R2) family signaling pathway in psoriasis via imiquimod(IMQ) treatment. Methods IL-20R2 knockdown(IL-20R2^-/-) mice and wild type IL-20R2^+/+ mice were used in this experiment. IL-20R2 mRNA in the skin tissue of the mice was detected by PCR sequencing and the IL-20R2 protein level was analyzed by Western blot analysis. We then established a psoriasis-like mouse model by topical treatment with IMQ, and body mass and skin lesions were recorded daily. In addition, HE staining was performed to observe the pathological changes of the skin. Results Compared with wild type IL-20R2^+/+ mice, the IL-20R2^-/- mice showed lower levels of both IL-20R2 protein and mRNA in the skin tissue, also exhibited a markedly less pathological changes after IMQ induction. Conclusion Knockdown of IL-20R2 gene can inhibit the development of psoriasis induced by IMQ in mice.