c-FLIP基因在青海地区藏族、汉族、回族3个民族宫颈癌组织中表达的差异性及意义

Difference of c-FLIP gene expression in cervical cancer of women of Tibetan nationality,Han nationality,and Hui nationality in Qinghai and the significance

目的探讨c-FLIP基因在青海地区藏族、汉族、回族3个民族宫颈癌组织中表达的差异性及其意义,为临床诊治提供参考依据.方法收集青海地区藏族、汉族、回族3个民族宫颈癌组织蜡块各20例,共60例,SP免疫组化方法检测c-FLIP在3个民族宫颈癌组织中的表达水平,并对各民族病例c-FLIP阳性率进行统计分析;对3个民族宫颈癌新鲜手术标本进行c-FLIPmRNA表达水平的检测.结果c-FLIP蛋白检测显示汉族阳性率为65.0%,其中强阳性率为45.0%;藏族阳性率为65.0%,其中强阳性率为25.0%;回族阳性率为40.0%,其中强阳性率占10.0%;mRNA表达水平显示c-FLIP在不同民族的宫颈癌组织和癌旁组织均有表达,在汉族宫颈癌组织表达量最高,藏族次之,回族最低;3个民族的癌旁组织也有一定量c-FLIP表达,但藏族最高;3个民族宫颈癌癌组织c-FLIP表达均高于癌旁组织;不同年龄段3个民族c-FLIP表达呈现不同表达水平.结论不同民族整体c-FLIP表达存在一定差异性,不同民族癌组织c-FLIP表达均高于癌旁组织,提示c-FLIP可能与宫颈癌的发生发展有一定的关系.但是同一年龄段不同民族c-FLIP表达差异性尚不能确定.

Objective To explore the difference of c-FLIP gene expression in cervical cancer of women of Tibetan nationality,Han nationality,and Hui nationality in Qinghai and the significance,provide a reference basis for clinical diagnosis and treatment. Methods Sixty paraffin-embedded cervical cancer tissue samples were collected in Qinghai,including 20 samples of Tibetan women,20 samples of Han women,and 20 samples of Hui women. Immunohistochemical SP method was used to detect the expession levels of c-FLIP in the three groups,the positive rates of c-FLIP were calculated. c-FLIP m RNA levels were detected in fresh surgical specimens of women in the three groups. Results The positive rate and strong positive rate of c-FLIP protein in Han women were 65. 0% and 45. 0%,respectively;The positive rate and strong positive rate of c-FLIP protein in Tibetan women were 65. 0% and 25. 0%,respectively;The positive rate and strong positive rate of c-FLIP protein in Hui women were 40. 0% and 10. 0%,respectively. c-FLIP m RNA expressed in cervical cancer tissue and cervical tissue adjacent to cancer in Han,Tibetan,and Hui women,the expression level was the highest in Han women,followed by Tibetan women,which was the lowest in Hui women. c-FLIP m RNA expressed in cervical tissue adjacent to cancer in Han,Tibetan,and Hui women to a certain extent,and the expression level was the highest in Tibetan women. The expression levels of c-FLIP in cervical cancer tissue were higher than those in cervical tissue adjacent to cancer in Han,Tibetan,and Hui women. The expression levels of c-FLIP in Han,Tibetan,and Hui women in different age groups were different. Conclusion There is a certain difference in c-FLIP expression among the women of different nationalities,the expression levels of c-FLIP in cervical cancer tissue are higher than those in cervical tissue adjacent to cancer,which indicates that c-FLIP may be correlated with oncogenesis and aggravation of cervical cancer to a certain extent,but the difference of c-FLIP expression in Han,Tibetan,and Hui women in the same age group is uncertain.