Box-Behnken响应面法优选凤丹皮中苷类成分的醇提工艺

Box-Behnken Response Surface Methodology for Optimizing the Ethanol Extraction Process of Glycosides from Cortex Paeoniae Sinensis

目的优选凤丹皮中苷类成分的醇提工艺。方法在单因素试验的基础上,以乙醇浓度、料液比和提取时间3个因素为自变量,以氧化芍药苷、芍药苷、苯甲酰芍药苷和浸膏得率的综合评分为因变量,采用基于Box-Behnken设计的响应面法优化凤丹皮中苷类成分的醇提工艺。结果最优醇提工艺为60%乙醇,料液比1:70,提取2次,每次90min。结论优选的醇提工艺方法简单、稳定可行,可为进一步的生产开发和应用研究提供参考。

Objective To optimize the ethanol extraction process of glycosides from cortex paeoniae sinensis. Methods On the basis of single factor experiment, taking ethanol concentration, solid-liquid ratio and extraction time as independent variables, and taking the comprehensive scores of paeoniflorin oxide, paeoniflorin, benzoyl paeoniflorin and extract yield as dependent variables, response surface methodology based on Box-Behnken design was used to optimize the ethanol extraction process of glycosides from cortex paeoniae sinensis. Results The optimum ethanol extraction process was 60% ethanol, the ratio of material to liquid was 1:70, extraction twice, 90 minutes each time. Conclusion The optimized ethanol extraction process is simple, stable and feasible, which can provide reference for further production development and application research.