早期动脉粥样硬化患者的血清代谢组学研究

Serum metabolomics investigation of patients with early atherosclerosis

目的采用基于液相色谱-质谱联用技术的代谢组学方法,探讨早期动脉粥样硬化(AS)患者的血清生物标志物,为AS的发生机制和早期诊断提供依据。方法运用基于超高效液相色谱-四极杆飞行时间质谱技术对24例健康志愿者(对照组)和27例AS患者(AS组)的血清样本进行代谢组学分析。采用正交偏最小二乘法判别分析方法对所得数据进行模式识别,分析两组代谢谱差异,筛选并鉴定两组差异代谢产物。结果血清样本中共鉴定分析6个差异代谢产物,其中AS患者血清中甜菜碱含量升高(P<0.05),而磷脂酰胆碱等5个代谢产物的水平降低(P<0.05)。结论AS发生早期,机体氨基酸代谢、脂肪酸代谢及三羧酸循环等代谢通路发生改变,所鉴定代谢产物有望成为AS早期筛查指标。

Objective To investigate the serum metabolic profiles of early atherosclerosis in patients by LCMS- based method to provide the basis for early diagnosis. Methods Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was applied to process the serum samples of 24 healthy volunteers and 27 atherosclerosis patients. Orthogonal to partial squares discriminant analysis was carried out for pattern recognition and characteristic metabolites identification between the two groups. Results There were 6 biomarkers identified after multivariate statistical analysis. The level of betaine was increased and 5 biomarkers such as phosphatidylcholine were decreased in the serum of atherosclerosis patients (P < 0.05). Conclusions Based on the analysis of metabolic pathways, amino acid metabolism, fatty acid metabolism and tricarboxylic acid cycle in atherosclerosis patients are disordered. These 6 different biomarkers can be helpful for the early diagnosing of atherosclerosis.