血压控制良好的高血压3级患者内皮祖细胞数量及功能变化

The Number and Function of Endothelial Progenitor Cells in Patients with Grade 3 Hypertension which was well controlled

目的探究既往血压控制良好的高血压3级患者内皮祖细胞EPCs)数量和功能的变化。方法研究组为13例既往血压控制良好的高血压3级住院患者和13例既往血压控制良好的高血压1、2级住院患者,对照组为同期健康体检者20例。采集各组研究对象外周血20mL,采用Dil-ac-LDL及lectin荧光标记方法检测体外培养EPCs数量,CCK8法和Transwell小室检测EPCs功能(增殖、迁移)。结果研究组患者EPCs数量及功能均明显低于对照组,差异均有统计学意义(P<0.05)。高血压1、2级患者EPCs数量及功能与高血压3级患者比较,差异均无统计学意义(P>0.05)。结论高血压3级患者长期血压控制良好后EPCs功能及数量均能得以保留。

Objective To investigate the number and function of endothelial progenitor cells(EPCs) in patients with grade 3 hypertensive which was well controlled before. Methods 13 inpatients with grade 3 hypertension and grade 1 and 2 hypertension which were well controlled before were enrolled in this study,and 20 healthy persons were recruited as control group.The number of EPCs cultured in vitro was detected by Dil-ac-LDL and Lectin fluorescence labeling,and the function of EPCs(proliferation and migration) were analyzed by CCK8 and Transwell chambers. Results Compared with the control group,the number and function(migration, proliferation) of EPCs in hypertensive inpatients were significantly lower( P <0.05).However,there was no significant difference in the number and function of EPCs between grade 1-2 hypertension patients and grade 3 hypertension patients( P >0.05). Conclusion The function and number of EPCs can be preserved after long-term baseline blood pressure well controlled in patients with grade 3 hypertension.