益气健脾化瘀祛痰方对脾虚痰浊动脉粥样硬化巴马小型猪探究行为的影响

Effects of Yiqi Jianpi Huayu Qutan Formula(益气健脾化瘀祛痰方) on the Exploring Behavioral of Bama Mini Pigs with Spleen Deficiency and Phlegm Turbidity Syndrome of Atherosclerosis

目的观察益气健脾化瘀祛痰方对脾虚痰浊动脉粥样硬化(AS)巴马小型猪探究行为的影响。方法将26只巴马小型猪随机分为正常组5只、手术组21只,正常组给予基础饲料,手术组给予高脂饲料喂饲。第2周对手术组小型猪行冠状动脉球囊挤压术,术后将21只小型猪随机分为模型组、中药组和西药组,每组7只。第4周开始对除正常组外的各组小型猪加入跑步过劳训练制备脾虚痰浊AS模型,共持续4周。24周后评价模型,评价后中药组给予益气健脾化瘀祛痰方1.625 g/(kg·d),西药组予单硝酸异山梨酯缓释片1.142 mg/(kg·d)。24周后采用监视仪器对各组小型猪咬物品、咬同伴、拱物品、拱同伴、闻物品、闻同伴6项探究行为进行观察。结果与正常组比较,模型组小猪探究行为中咬同伴、拱物品行为频次下降,差异有统计学意义(P<0.05);与模型组比较,中药组和西药组探究行为频次均呈上升趋势,但差异无统计学意义(P> 0.05);中药组在咬物品、拱物品、闻物品方面频次多于西药组,其中咬物品差异有统计学意义(P<0.05)。结论益气健脾化瘀祛痰方可增加脾虚痰浊AS小型猪探究行为活动,且对小型猪探究行为改善作用较单硝酸异山梨酯缓释片更显著。

Objective To observe the effects of Yiqi Jianpi Huayu Qutan Formula(益气健脾化瘀祛痰方) on the exploring behavioral of Bama mini pigs with spleen deficiency and phlegm turbidity syndrome of atherosclerosis(AS).Methods A total of 26 Bama mini pigs were divided into two group,i.e.5 in a normal group and 21 in a surgical group.The normal group was given a basic diet and the surgical group was given high fat diet.The mini pigs of the surgical group underwent coronary balloon compression with strain in the 2 nd week.21 mini pigs were randomly divided into a model group,a Chinese medicine group and a western medicine group,with 7 pigs in each group.The running overwork training for mini pigs in each operation group from the 4 th week was added to establish AS with spleen deficiency and phlegm turbidity syndrome model,except for the normal group,for 4 weeks.After 24 weeks,the model was evaluated.The Chinese medicine group was given Yiqi Jianpi Huayu Qutan Formula(1.625 g/kg/d).The western medicine group was fed isosorbide mononitrate sustained-release tablets 1.142 mg/(kg·d).After 24 weeks,the monitoring instruments were used to observe the exploring behaviors of the mini pigs,biting companions,arching items,arching companions,smelling articles,and smelling companions.Results Compared with the normal group,the frequency of biting companions and arching items in the model group was decreased,and the difference was statistically significant(P<0.05).Compared with the model group,the frequency of exploring behaviors of the Chinese medicine group and the western medicine group showed a rising trend.However,the difference was not statistically significant(P > 0.05).The Chinese medicine group was more frequent than the western medicine group in biting,arching and smelling items,and the difference of biting items was statistically significant(P<0.05).Conclusion Yiqi Jianpi Huayu Qutan Formula can increase exploring behavioral activities of AS mini pigs with the spleen deficiency and phlegm turbidity syndrome,and the improvement of exploring behavior of mini pigs is more significant than that of isosorbide mononitrate sustained-release tablets.