辣木叶及其复方对便秘大鼠的通便作用和胃肠激素水平的影响

Influence of Moringa oleifera and Moringa oleifera Purgative Compound on improving defecation and gastrointestinal hormones in constipated rats

目的观察辣木叶及其复方对便秘模型大鼠的通便作用和相关胃肠激素水平的影响。方法按体质量随机将100只SD大鼠分为10组,即对照组、模型组、番泻叶浸液组(阳性对照,0.01 g/kg)和辣木叶水提液低、中、高剂量(0.79、1.58、3.15 g/kg)组,黄芪白术(1.05 g/kg)组,辣木叶复方水提液低、中、高剂量(1.84、2.63、4.20 g/kg)组。除对照组外,均采用盐酸洛哌丁胺(6.67 mg/kg)复制便秘模型,每日2次,连续2周。造模结束后,各给药组分别ig相应受试样品,每天1次,连续7 d。记录各组大鼠体质量、最后24 h粪便粒数,测定粪便含水量、小肠炭末推进率,ELISA试剂盒法检测血清P物质、生长抑素含量及结肠胃动素、血管活性肠肽含量。结果与模型组比较,辣木叶水提液中、高剂量组及辣木叶复方水提液各剂量组的体质量增量显著升高(P<0.05),各给药组大鼠的排便粒数与模型组比较均显著性增加(P<0.05),各给药组大鼠粪便含水量与模型组比较均无显著性差异,辣木叶水提液中、高剂量组及其复方水提液中、高剂量组小肠炭末推进率显著增加(P<0.05、0.01)。辣木叶水提液中、高剂量组及其复方水提液各剂量组血清P物质含量、结肠组织胃动素含量均显著高于模型组(P<0.05、0.01),辣木叶水提液高剂量组及辣木叶复方水提液中、高剂量组大鼠血管活性肠肽含量较模型组降低显著(P<0.05、0.01),各给药组大鼠血清生长抑素水平均显著低于模型组(P<0.01)。辣木叶复方水提物上述作用均好于等剂量辣木叶水提液及黄芪白术水提液。结论辣木叶及其复方具有良好的通便作用,可能与调节胃肠激素水平有关,复方的通便及调节胃肠激素的作用优于单味药辣木叶及黄芪白术,体现出复方配伍的整体优越性,其中辣木叶侧重调节P物质、胃动素及血管活性肠肽水平,而黄芪白术主要调节生长抑素水平。

Objective To observe the impacts of Moringa oleifera and Moringa oleifera Purgative Compound on defecation and gastrointestinal hormones in rats with constipation.Methods A total of 100 SD rats with half male and half female were randomly divided into ten groups:control group,model group,Senna leaf extract group(positive control,0.01 g/kg),Water extract of Huangqi Baizhu group,water extract of M.oleifera(WEMO) low,medium,high dose(0.79,1.58,and 3.15 g/kg) groups and water extract of M.oleifera Purgative Compound(WEMOPC) low,middle,high dose(1.84,2.63,and 4.20 g/kg) groups.Loperamide hydrochloride was administered at a dose of 6.67 mg/kg per day by ig for continuous two weeks so that constipation model was established.Each group was treated with corresponding drugs respectively,once a day for seven days.After one week,body weight,fecal number and water content within 24 h,intestinal propulsive rate,contents of motilin,substance P,vasoactive intestinal peptide and somatostatin were measured.Results Compared with the model group,the body weight increment of WEMO medium and high dose group and WEMOPC each dose group increased significantly(P<0.05);the fecal granule number of rats in each dose group increased significantly compared with the model group(P<0.05);the fecal water content of rats in each dose group had no significant difference compared with the model group;the small intestinal carbon powder propulsive rate of WEMO medium and high dose group and its compound water extract medium and high dose group increased significantly(P<0.05,0.01).The contents of substance P in serum and motilin in colon tissue in WEMO medium and high dose group and its compound water extract groups were significantly higher than those in model group(P<0.05 and 0.01);The contents of vasoactive intestinal peptide in WEMO high dose group and WEMOPC medium and high dose group were significantly lower than those in model group(P<0.05 and0.01);The levels of somatostatin in serum of rats in each treatment group were significantly lower than those in model group(P<0.01).The above effects of WEMOPC were better than those of the same dose of WEMO and water extract of Huangqi Baizhu group.Conclusion M.oleifera and its compound have good defecation effect,which may be related to the regulation of gastrointestinal hormone level.The effect of the compound on defecation and regulation of gastrointestinal hormone is better than that of the single herb M.oleifera and Huangqi Baizhu,reflecting the overall superiority of the combination.M.oleifera leaves mainly regulate the levels of substance P,motilin and vasoactive intestinal peptide,while Huangqi Baizhu mainly regulates the level of somatostatin.