海马补肾丸免疫调节及抗应激作用的实验研究

Experimental study on immune regulation and anti-stress effect of Haima Bushen Pills

目的采用不同模型评价海马补肾丸免疫调节作用和抗应激作用。方法健康昆明种小鼠随机分为对照组、模型组、香菇菌多糖(100 mg/kg)组、龟龄集(0.24 g/kg)组和海马补肾丸0.55、1.10、2.20 g/kg组,每天ig给药1次,连续14 d。①采用注射盐酸环磷酰胺致小鼠免疫抑制模型,给药结束尾iv 25%印度墨汁,计算廓清指数(κ)和吞噬指数(α),观察小鼠肝指数、脾指数和胸腺指数,②采用注射盐酸环磷酰胺致小鼠免疫抑制模型,ip 5%鸡红细胞悬液0.3 mL致敏,观察各组血清溶血素生成量,③给药第8~10天,除对照组外,剩余各组开始ip环磷酰胺80 mg/kg,连续3 d,继续给药,至给药第14天眼眶静脉取血,测定白细胞数及免疫球蛋白IgG、IgM、IgA含量,④SD大鼠随机分为对照组、模型组、单硝酸异山梨酯片20 mg/kg、龟龄集0.12 g/kg和海马补肾丸低、中、高剂量(0.28、0.56、1.12 g/kg)组,每天ig给药1次,连续14 d,观察海马补肾丸对运动疲劳大鼠游泳时间及运动后对血清尿素氮(BUN)、乳酸(LD)、肝糖原水平的影响,⑤健康昆明种小鼠随机分为对照组、模型组、单硝酸异山梨酯片(40 mg/kg)组、龟龄集(0.24 g/kg)组和海马补肾丸低、中、高剂量(0.55、1.1、2.2 g/kg)组,每天ig给药1次,连续5 d,于末次给药后1 h,采用注射异丙肾上腺素致小鼠缺氧模型,将小鼠置于密封瓶中,观察存活时间。结果与模型组比较,海马补肾丸2.2 g/kg显著增加α及κ(P<0.05、0.01),海马补肾丸1.1、2.2 g/kg剂量显著增加脾、胸腺系数(P<0.05、0.01),海马补肾丸各剂量组显著增加血清溶血素生成量(P<0.05、0.01),海马补肾丸2.2 g/kg剂量组显著增加白细胞数量及免疫球蛋白IgG、IgM、IgA的含量(P<0.05),海马补肾丸0.56、1.12 g/kg剂量显著延长大鼠力竭游泳时间(P<0.05、0.01),1.12 g/kg剂量能显著地减少血清中BUN、LD含量,减少肝糖原消耗(P<0.05、0.01),海马补肾丸1.1、2.2 g/kg剂量能显著延长小鼠缺氧存活时间(P<0.05、0.01)。结论海马补肾丸具有明显免疫增强和抗应激作用。

Objective Different models were used to evaluate the immunomodulatory effect and anti-stress effect of Haima Bushen Pills.Methods The healthy Kunming mice were randomly divided into control group,model group,lentinan group(100 mg/kg),Guilingji group(0.24 g/kg) and Haima Bushen Pills group(0.55,1.10 and 2.20 g/kg).The mice were ig given relevant drugs once a day for 14 days.① Immunosuppressive model of mice was induced by cyclophosphamide hydrochloride injection.Indian ink of25% was iv at the end of administration.Clearance index(κ) and phagocytic index(α) were calculated.Liver index,spleen index and thymus index of mice were observed.② Immunosuppressive model of mice was induced by injection of cyclophosphamide hydrochloride,and sensitized by 0.3 mL of IP 5% chicken erythrocyte suspension.Serum hemolysin production was observed in each group.③ On the 8 th to 10 th day of administration,except the control group,the remaining groups began ip cyclophosphamide80 mg/kg for 3 consecutive days.The orbital vein blood was collected on the 14 th day of administration,and the number of white blood cells and the contents of immunoglobulin IgG,IgM and IgA were measured.④ SD rats were randomly divided into control group,model group,isosorbide mononitrate tablets 20 mg/kg,Guilingji 0.12 g/kg and low,medium and high dose groups(0.28,0.56,1.12 g/kg) of Hippocampus Kidney-tonifying Pills.The effects of Hippocampus Kidney-tonifying Pills on the levels of serum urea nitrogen(BUN),lactic acid(LD) and hepatic glycogen in exercise-fatigue rats were observed once a day for 14 days.⑤Healthy Kunming mice were randomly divided into control group,model group,isosorbide mononitrate tablet(40 mg/kg),Guilingji(0.24 g/kg) group and Hippocampus Kidney-tonifying Pills(0.55,1.1,2.2 g/kg) group.The mice were given Ig once a day for 5 days.One hour after the last administration,the mice were injected with isoprenaline to induce hypoxia.The mice were placed in sealed bottles for observation of survival time.Results Compared with model group,2.2 g/kg of Haima Bushen Pills significantly increased α and κ(P<0.05 and 0.01),1.1 and 2.2 g/kg of Haima Bushen Pills significantly increased spleen and thymus coefficient(P<0.05 and 0.01),and each dose of Haima Bushen Pills significantly increased serum hemolysin production(P<0.05 and 0.01);2.2 g/kg of Haima Bushen Pills significantly increased the number of white blood cells and the IgG,IgM and IgA(P<0.05);the dosage of Haima Bushen Pills(0.56,1.12 g/kg) significantly prolonged the exhaustive swimming time of rats(P<0.05,0.01);the dosage of 1.12 g/kg could significantly reduce the contents of BUN and LD in serum and the consumption of hepatic glycogen(P<0.05,0.01);the dosage of Haima Bushen Pills(1.1,2.2 g/kg) could significantly prolong the hypoxic survival time of mice(P<0.05,0.01).Conclusion Haima Bushen Pills has obvious immune enhancement and anti-stress effect.