响应面法优化壳聚糖对三七茎叶皂苷提取液除杂工艺的影响

Effects of Purifying Technology of the Saponin Extract of Panax notoginseng Leaves and Stems with Chitosan by Response Surface Methodology

在单因素分析的基础上,以药液质量体积比、壳聚糖用量和作用温度为自变量,以固形物去除率和皂苷保留率为评价指标,设计了三因素三水平响应面法优化试验,研究壳聚糖用于三七茎叶皂苷提取液除杂的最佳工艺。结果表明,三七茎叶皂苷提取液除杂最佳工艺:药液质量体积比[生药质量(g)∶提取液体积(mL)]为1∶6,壳聚糖(1%溶液)用量(壳聚糖溶液体积与生药量之比)为0.8 mL/g,作用温度为65℃。三七茎叶皂苷提取液除杂工艺优化后,固形物去除率及皂苷保留率分别为26.09%、96.10%,其RSD分别为1.54%、0.69%,重现性较好,表明该模型有效。

In order to determine the optimum edulcoration process of the saponin extract of leaves and stems of Panax notoginseng,based on single factor analysis,the mass-volume ratio of medicine to extract liquid,the chitosan dosage,and acting temperature as independent variables,and the solid removal rate and saponin retention rate as the evaluation indexes,experiments with three factors and three levels of response surface methodology were established.The results showed that the best purification process of the saponin extract of leaves and stems of Panax notoginseng was as follows: The mass-volume ratio of medicine to extract liquid[the ratio of the quality of raw medicine(g)to the volume of the extract liquid(mL)]was 1∶6,the dosage of 1% chitosan solution(the ratio of the volume of chitosan solution to the quantity of raw medicine)was 0.8 mL/g,and the acting temperature was 65 ℃.Under this condition,the saponin extract of leaves and stems of Panax notoginseng was purified and experiments were repeated three times.The results showed that the solid removal rate and saponin retention rate were 26.09% and 96.10%,respectively,the RSDs were 1.54% and 0.69%,respectively.The repeatability was good,which verified the validity of this model.