北京地区枣疯病植原体潜在介体叶蝉种类筛查

Leafhopper species screening of potential vector transmitting jujube witches’-broom phytoplasma in Beijing

【目的】筛查北京地区枣园感染枣疯病植原体的叶蝉种类,确定枣疯病植原体潜在介体昆虫,为预防和控制枣疯病流行提供科学依据。【方法】通过扫网法和黄板诱集法,对昌平区流村镇流村、海淀区西北旺镇唐家岭村、朝阳区奥林匹克森林公园、通州区永于路北京观光南瓜园4个地点枣树上叶蝉种类及枣疯病植原体感染的叶蝉进行调查和分子检测分析。【结果】北京地区枣园枣树上有13种叶蝉发生,其中包括片突菱纹叶蝉( Hishimonus lamellatus Cai et Kuoh)、凹缘菱纹叶蝉( H. sellatus (Uhler))、小绿叶蝉( Empoasca spp.)、斑叶蝉( Erythroneura sp.)、横带叶蝉( Scaphoideus festivus Matsumura)、大青叶蝉( Cicadella viridis (Linnaeus))、新县长突叶蝉( Batracomorphus xinxianensis Cai et Shen)、红闪小叶蝉( Zygina sp.)、白边大叶蝉( Kolla paulula (Walker))、桃一点叶蝉( Singapora shinshana (Matsumura))、一点木叶蝉( Phlogotettix cyclops (Mulsant et Rey))、条沙叶蝉( Psammotettix striatus (Linnaeus))和窗耳叶蝉( Ledra auditura Walker)。通过对采集的叶蝉标本采用植原体通用引物进行PCR检测,片突菱纹叶蝉( H. lamellatus )、凹缘菱纹叶蝉( H. sellatus )、大青叶蝉( C. viridis )、白边大叶蝉( K. paulula ),均发现感染枣疯病植原体,感染率分别为1.4%、3.0%、7.4%、8.3%,其他叶蝉经检测未发现感染植原体。【结论】凹缘菱纹叶蝉( H. sellatus )、片突菱纹叶蝉( H. lamellatus )、大青叶蝉( C. viridis )、白边大叶蝉( K. paulula )可能为潜在的枣疯病植原体介体昆虫。

【Objective】 Leafhopper speceis screening of potential vector transmitting jujube witches’-broom phytoplasma is the basis for managing jujube witches’-broom disease.【Methods】 The leafhopper populations on jujubes were collected using yellow sticky traps and net capture in Liucun (Changping District, Beijing), Tangjialing (Haidian District, Beijing), Olympic Forest Park (Chaoyang District, Beijing), and Sightseeing Pumpkin Garden (Tongzhou District, Beijing). Molecular detection and analysis were performed to identify the infection of witches’-broom phytoplasma.【Results】 A total of 13 leafhopper species were trapped on jujubes in Beijing, including Hishimonus lamellatus Cai et Kuoh, Hishimonus sellatus (Uhler), Empoasca spp., Erythroneura sp., Scaphoideus festivus Matsumura, Cicadella viridis (Linnaeus), Batracomorphus xinxianensis Cai et Shen, Zygina sp., Kolla paulula (Walker), Phlogotettix cyclops (Mulsant et Rey), Singapora shinshana (Matsumura), Psammotettix striatus (Linnaeus) and Ledra auditura Walker. PCR analysis showed that H. lamellatus , H. sellatus , C. viridis and K. paulula were infected with jujube witches’-broom disease phytoplasma, whereas other leafhopper species were free from phytoplasmas. In addition, the infection rates of these four infected species were 1.4%, 3.0%, 7.4%, and 8.3%.【Conclusion】 H. sellatus , H. lamellatus , C. viridis and K. paulula , might be other three potential jujube witches’-broom disease phytoplasma vector insects in Beijing.