葛根素对妊娠期糖尿病大鼠糖脂代谢作用的研究

Effect of Puerarin on Glucose and Lipid Metabolism in Gestational Diabetic Mellitus Rats

目的观察葛根素对妊娠期糖尿病大鼠糖脂代谢的影响.方法选用高脂喂养的妊娠d1的Wistar大鼠,予链脲佐菌素(STZ)25 mg/kg一次性腹腔注射,注射72 h后,空腹血糖稳定在6.67~16.67 mmol/L为GDM模型复制成功.将造模成功的大鼠随机分为模型组、葛根素组各6只,另取6只正常妊娠大鼠为正常对照组,从妊娠d1起葛根素组予以葛根素0.25 g/(kg·d)灌胃,正常组及模型组给予等容积0.9%的生理盐水灌胃,至产出子鼠.观察各组大鼠25周时血清空腹血糖(FBG)、空腹胰岛素(FBINS)、血脂、肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)、瘦素(Leptin)、脂联素(APN)的变化情况,qPCR检测肝脏组织中瘦素受体、胰岛素受体及脂肪组织中TNF-α、IL-6、APN、Leptin mRNA的表达.结果葛根素组大鼠FBG、甘油三酯(TG)、低密度脂蛋白(LDL-C)、总胆固醇(TC)、TNF-α、IL-6、Leptin与模型组比较均有明显下降(P<0.05~0.01),葛根素组大鼠高密度脂蛋白(LDL-H)、APN水平与模型组比较有显著上升(P<0.05~0.01),葛根素组大鼠脂肪组织中胰岛素受体mRNA的表达较模型组显著增加,葛根素组大鼠肝脏组织中TNF-α、Leptin mRNA的表达较模型组明显下降(P<0.05).结论葛根素可改善妊娠期糖尿病大鼠糖脂代谢,其作用机制可能与调节TNF-α、Leptin、APN水平相关.

OBJECTIVE To observe the influence of puerarin on glucose and lipid metabolism in gestational diabetic mellitus(GDM) rats. METHODS High fat feeding pregnant wistar rats were given streptozocin (STZ) 25 mg/kg intraperitoneally on the first day of pregnant. After injection for 72 hours, rats with FBG between 6.67 16.67 mmol/L were gestational diabetic models, which were randomly divided into two groups: GDM model group(MG, using 0.9% NaCl), puerain treated group(TG, using puerain 0.25 g/(kg·d), 6 rats in each group. And we selected 6 pregnant rats as normal gestation group(NG, using 0.9% NaCl). The rats were given drugs by intragastric administration until the offspring were born. The levels of FBG, insulin, serum lipid, TNF-α, IL 6, leptin and adiponection were observed at the age of 25 weeks. Leptin receptor insulin receptor, TNF-α, IL-6, adiponection and leptin mRNA expression in fat and liver tissues were detected by qPCR. RESULTS Compared with GDM model group, the levels of FBG, insulin, TG, TC, LDL-C, TNF-α, IL-6 and leptin of puerarin treated group were significantly decreased(P<0.05,P<0.01). The levels of HDL-C and adiponection of puerarin treated group were significantly increased(P<0.05,P<0.01). The mRNA expression of IR in TG was higher than MG, and the mRNA expression of TNF-α and leptin in TG were decreased significantly (P<0.05). CONCLUSION Puerarin can improve glucose and lipid metabolism in gestational diabetic rats, the mechanism may be related to the regulation of TNF-α, IL-6, leptin and adiponectin levels.