全氟化碳对脂多糖致A549细胞损伤划痕愈合能力和凋亡基因表达水平的影响

Effect of perfluorocarbon on the scratch healing ability in A549 cells induced by lipopolysaccharide and the expression level of apoptotic genes

目的探讨全氟化碳(PFC)对脂多糖(LPS)致A549细胞损伤划痕愈合能力和凋亡基因表达水平的影响,为其在急性呼吸窘迫综合征临床治疗中的应用提供理论依据。方法以常规传代培养的人肺上皮样A549细胞为研究对象,分为四组:对照组不予以任何处理,PFC组按PFC:培养液=1:10的体积比加入PFC,LPS组加入终点浓度为100μg/mL的LPS,PFC组+LPS组加入按上述两组方法加入PFC及LPS。行划痕愈合实验,计算划痕愈合率及划痕边缘细胞核间距;并应用流氏细胞仪检测细胞凋亡情况。结果划痕愈合实验显示,与对照组相比,LPS组及LPS+PFC组划痕愈合率均明显降低(P<0.05),划痕边缘细胞核间距明显减小(P<0.05);且与LPS组相比,LPS+PFC组划痕愈合率明显提高(P<0.05),划痕边缘细胞核间距明显增大(P<0.05)。细胞凋亡检测显示,与对照组相比,LPS组及LPS+PFC组划细胞凋亡率均明显增高(P<0.05);且LPS+PFC组细胞凋亡率明显低于LPS组(P<0.05)。结论PFC能够促进LPS致A549细胞损伤的修复,抑制LPS诱导的A549细胞凋亡。

Objective To investigate the effect of perfluorocarbon (PFC) on the scratch healing ability in A549 cells induced by lipopolysaccharide(LPS) and the expression of apoptotic genes,in order to provide a theoretical basis for its application in the clinical treatment of acute respiratory distress syndrome.Methods Human lung epithelial A549 cells in conventional subculture were taken as the study objects,and they were divided into four groups:the control group was not treated at all,the PFC group was added to PFC according to the volume ratio of PFC/ nutrient solution with 1:10,the LPS group was added to the LPS at a end point concentration of 100 μg/mL,and the PFC combined with LPS group were added with PFC and LPS according to the above two methods.The scratch healing experiment was carried out,and the scratch healing rate and the nuclear spacing of the edge of the scratch were calculated.The cell apoptosis was detected by flow cytometry.Results The scratch healing test showed that the scratch healing rate of the LPS group and the LPS combined with PFC group was significantly lower than that of the control group ( P< 0.05).The nuclear spacing of the edge of scratch was significantly reduced ( P< 0.05).Compared with the LPS group,the scratch healing rate of the LPS combined with PFC group increased significantly ( P< 0.05),and the internuclear spacing of the scratched margin also increased significantly ( P< 0.05).Apoptosis assay showed that the apoptosis rate of the LPS group and the LPS combined with PFC group was significantly higher than that of the control group ( P< 0.05).The apoptosis rate of the LPS combined with PFC group was significantly lower than that of the LPS group ( P< 0.05).Conclusion PFC can promote the repair of A549 cells induced by LPS and inhibit the apoptosis of A549 cells induced by LPS.