奥曲肽对胰腺癌模型大鼠细胞凋亡的影响及作用机制研究

Effect of octreotide on apoptosis of pancreatic cancer model rats and its mechanism

目的探究奥曲肽对胰腺癌模型大鼠细胞凋亡的影响及相关作用机制。方法选取50只SD健康雄性大鼠,随机选取10只作为正常组,其余40只大鼠进行胰腺癌模型建立,并将胰腺癌模型大鼠随机分为模型组以及低、中、高剂量奥曲肽组,正常组、模型组大鼠使用生理盐水进行干预,低、中、高剂量奥曲肽组大鼠使用不同剂量奥曲肽进行干预。对比各组大鼠肿瘤体积、质量以及肿瘤细胞凋亡、周期分布情况,Western blot法检测Bcl-2、PI3K、Akt、PTEN、p-Akt、mTOR表达。结果高剂量奥曲肽组大鼠肿瘤体积、质量均低于低、中剂量奥曲肽组(P均<0.05)。高剂量奥曲肽组大鼠肿瘤细胞凋亡率高于低、中剂量奥曲肽组(P均<0.05)。高剂量奥曲肽组处于G1期的细胞比例高于低、中剂量奥曲肽组(P均<0.05)。高剂量奥曲肽组Bcl-2、PI3K、Akt、p-Akt、mTOR相对表达量均低于低、中剂量奥曲肽组(P均<0.05);PTEN相对表达量高于低、中剂量奥曲肽组(P均<0.05)。结论使用较高剂量奥曲肽对胰腺癌模型大鼠进行干预,能够使大鼠肿瘤体积、质量下降,调控细胞凋亡相关蛋白Bcl-2、PI3K、Akt以及周期相关蛋白PTEN、p-Akt、mTOR的表达,从而促进细胞凋亡,改善细胞周期分布,为胰腺癌的治疗提供理论帮助。

Objective To explore the effect of octreotide on apoptosis of pancreatic cancer model rats and its related mechanism. Methods Fifty healthy male SD rats were randomly selected as normal group, and the remaining 40 rats were used to establish pancreatic cancer model. The pancreatic cancer model rats were randomly divided into model group and low, medium and high dose octreotide group. Normal and model rats were intervened with normal saline, while low, medium and high dose octreotide groups were intervened with different doses of octreotide. Western blot was used to detect the expression of Bcl-2, PI3K, Akt, PTEN, p-Akt and mTOR. Results The tumor volume and weight of the high-dose octreotide group were lower than those of the low-dose and medium-dose octreotide group ( P <0.05). The apoptosis rate of tumor cells in high-dose octreotide group was higher than that in low-dose and medium-dose octreotide group ( P <0.05). The proportion of cells in G1 phase in the high-dose octreotide group was higher than that in the low-dose and medium-dose octreotide group ( P <0.05). The relative expression levels of bcl-2, PI3K and Akt in the high-dose octreotide group were lower than those in the low-dose and medium-dose octreotide group ( P <0.05). The relative expression levels of PTEN in the high-dose octreotide group were higher than those in the low-dose and medium-dose octreotide group, and the relative expression levels of p-akt and mTOR were lower than those in the low-dose and medium-dose octreotide group ( P <0.05). Conclusion The intervention of high dose octreotide on pancreatic cancer model rats can reduce the volume and weight of the tumors, regulate the expression of Bcl-2, PI3K, Akt, PTEN, p-Akt and mTOR, so as to promote cell apoptosis, improve cell cycle distribution and provide theoretical help for the treatment of pancreatic cancer.