银杏内酯B治疗缺血性脑中风的神经保护作用及机制的研究

Ginkgolide B Treatment for Ischemic Stroke Confers Neuroprotection and Its Mechanism

目的验证银杏内酯B(GB)可改善缺血性脑中风远期神经功能缺失,白质修复作用是否参与其中。方法成年小鼠随机分为Sham组、PBS组和GB组,后两组制作大脑中动脉栓塞模型,并予脑室给药干预。行为学检测神经功能,标记并统计MBP/SMI-32荧光比值以检测髓鞘修复程度。结果脑室予GB可降低损伤21d后患侧错步率,延长转棒时间(P<0.01)。标记并计算损伤35d髓鞘MBP/SMI-32值,GB组较PBS组明显增高(P<0.05)。结论GB对MCAO小鼠远期白质的修复作用参与了其神经功能的改善作用。

Objective To check Ginkgolide B(GB)Treatment for Ischemic Stroke Confers Neuroprotection and its white matter repair Mechanism. Methods Male mice subjected to MCAO after random allocation as sham, PBS or GB group. Latter two groups received intracerebroventricular injection of PBS or GB for 7 days. Neurobehavioral assessments and MBP/SMI-32 was detected. Results Consecutive GB infusion after MCAO obviously promoted recovery of neuronal ability in long-term period (P <0.01). Beneficial up-regulation of MBP/SMI-32 rate was also induced in sharp contrast with PBS group (P <0.05). Conclusion GB-improved white matter repair was involved in neurological recovery after MCAO.