基于UFLC-Triple TOF MS/MS技术分析竹节参中皂苷类成分

Saponins in Panacis japonici Rhizoma as Analyzed by UFLC-Triple TOF MS/MS

采用超快速液相色谱-三重四极杆飞行时间质谱技术对竹节参中皂苷类成分进行分析。采用色谱柱SynergiTM Hydro-RP 100A(2.0 mm×100 mm,2.5μm),以0.1%甲酸-水(A)-0.1%甲酸-乙腈(B)为流动相进行梯度洗脱;在电喷雾负离子扫描模式下采集数据。根据高分辨质谱提供的准分子离子和碎片离子的精确分子质量信息,结合标准品对照与参考相关文献数据,初步鉴定出53种皂苷,包括18种原人参二醇型、21种原人参三醇型、10种齐墩果烷型和4种奥寇梯木醇型皂苷。本实验可为探索竹节参药材的药效物质基础和建立其药材品质的综合评价体系提供基础资料。

In this study, we analyzed the saponins of Panacis japonici Rhizoma by ultra-fast liquid chromatography coupled with triple quadrupole-time of flight tandem mass spectrometry(UFLC-Triple TOF MS/MS). The chromatographic separation was performed on a SynergiTM Hydro-RP 100 ?(2.0 mm × 100 mm, 2.5 μm) with gradient elution using a mobile phase comprised of 0.1% formic acid solution(A)-0.1% formic acid acetonitrile(B). MS data were collected in the negative ion mode. Based on the accurate molecular mass information of quasi-molecular and fragment ions provided by highresolution mass spectrometry combined with standard reference data and relevant literature data, a total of 53 saponins werefinally identified, including 18 glycosides of protopanaxadiol(PPD), 21 glycosides of protopanaxatriol(PPT), 10 glycosides of oleanolic acid and 4 glycosides of ocotillone. This study paves the way for exploring the pharmacodynamic material basis of P. japonici Rhizoma and developing a comprehensive quality evaluation system.